7.
Quality
Control
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
113
7.4.4.4 Detection Limit ATP 1536-well Plate
Pipette the reagents into the wells of a Greiner 1536-well plate (white, flat bottom)
according to the Plate Layout
Material:
ATP Kit SL 144-041, BioThema AB
Greiner 384-well plate, flat bottom, white
10 µl p tips
Plate layout:
Pipette 10 µl of the Blank into the wells A1 – D1 and A3 – D10
Pipette 2 µl of ATP standard 10
-7
M into the wells A2 – D2, add 8 µl of ATP
reagent and mix in well (
use fresh tip for each well); ATP reagent must NOT
be contaminated with ATP standard!
<>
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
…
25
…
33
…
41
…
A
Bx
ATP
Bx
B
B
B
B
B
B
B
B
Bx
ATP
Bx
B
B
B
B
B
B
B
C
Bx
ATP
Bx
B
B
B
B
B
B
B
D
Bx
ATP
Bx
B
B
B
B
B
B
B
E
…
I
…
M
…
AA
…
AE
…
AI
…
AM
…
ATP….10 µl 2*10-8 M ATP; B….10 µl Blank, Bx…10 µl Blank used for crosstalk
check, not included in calculations below.
Measurement Parameters
Before pipetting the plate, prepare instrument for measurement:
Parameters:
Measurement mode:
Luminescence
Integration time:
1000 ms
Plate definition file:
GRE1536fw.pdfx
Part of the plate:
A1 – D10
Start measurement immediately after pipetting!
