6. Instrument Specifications
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
91
6.4 Fluorescence
Polarization
Parameters
Characteristics
Standard LED Excitation
470 nm, 530 nm, 590 nm, 635 nm
Emission Wavelength Range
280-850 nm, selectable in 1 nm steps
Emission Bandwidth
Selectable from 5 to 20 nm
Gain setting
see 6.3 Fluorescence Intensity and Time
Resolved Fluorescence (TRF)
6.4.1
Fluorescein 1nM (Fluorescence Polarization)
Plate type (number of wells)
384
1536
Dispensed Volume [µl]
100
10
Flashes per Well (1 flash = 10 ms
integration time)
10 10
Fluorescein 1 nM Precision [mP]
≤
2
≤
10
6.5 Absorbance
Parameters Characteristics
Wavelength range
230 - 1000 nm, selectable in 1 nm steps
Bandwidth Absorbance Scan
2.5 nm for meas.
≤
300 nm
5 nm
for meas. > 300 nm
Bandwidth Fixed Wavelength
5 nm
Measurement range
0 - 4 OD
Resolution 0.0001
OD
Accuracy (0-2 OD)
≤
± (1 % + 6 mOD)*
Precision (0-2 OD)
≤
± (0.5 % + 5 mOD)*
Linearity (0-2 OD)
R2
≥
0.999*
*Specifications valid only for 384-well plates.