5. Operating the INFINITE M1000 PRO
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
59
Z-Optimization
Z-optimization is only available for FI Top and FP measurements with the
INFINITE M1000 PRO
. For a particular assay, this procedure should be
performed once to determine the optimum working distance between the sample
in the plate and the fluorescence optics.
The z-position can be determined as follows:
(1)
‘Manual’
:
When using the ‘manual’ option, a numeric z-position value can be entered in the
measurement stripe.
(2)
‘Calculated from well’
:
When using the option ‘calculated from well’, the
INFINITE M1000 PRO
will
automatically identify the z-position of maximum signal in the selected well for
further measurements.
(3)
‘Same as’
for multi-labeling measurements:
When using the ‘same as’ option, the
INFINITE M1000 PRO
will automatically
use the same z-position as for a previously defined label, e.g. in a script with 2 FI
Top labels named as Label 1 and Label 2 the z-positionof Label 1 can also be
used for Label 2 by selecting the option‘Same as = Label 1’.
Select ‘
Z-Position
’ from the
Instrument
menu:
When using the ‘
Z-position
’ function in the instrument menu, the user can
determine the appropriate z-position from a graphical plot that shows the well(s)
used for z-positioning. The selected value is applied for further measurements.
Select the label(s) for which the z-position optimization is to be performed. The
optimal z-position can be simultaneously determined for up to 5 labels. The label
selection/number of labels depends on the measurement script previously
defined in i-control.
Figure 30
