4. Optical System
48
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
Fibers
A fiber guides the light from the sample to the detection unit. Three different fibers
are available for measuring 96, 384 or 1536-well plates.
The orifices in the ceiling of the measurement chamber are designed to receive
as much light as possible from the wells of 96, 384 or 1536-well plates, thus
maximizing the luminescence signal; however, the orifices do not receive
substantial amounts of light from neighboring wells, thereby minimizing crosstalk.
Filter Wheel
Two additional filters in the luminescence filter wheel allow for measurements of
AlphaScreen and AlphaLISA assays.
The transmission spectra of the AlphaScreen and AlphaLISA filters are shown in
figures 27 and 28.
0
10
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100
500
530
560
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680
Wavelength [nm]
T
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%
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Figure 27: Transmission spectrum of filter ‘AlphaScreen’.
0
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40
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500
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650
680
Wavelength [nm]
T
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[
%
]
Figure 28: Transmission spectrum of filter ‘AlphaLISA’.
Note
The luminescence attenuation and color filters installed in the filter
wheel can only be used for luminescence measurements, not for
AlphaScreen/AlphaLISA measurements.
