5. Operating the INFINITE M1000 PRO
72
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
The dead volume of the injection system (injector needles, syringes, valves and
tubing) is approximately 100 µl after using
‘backflush’
to return any unused
reagent to the reservoir bottles. The injection speed can be adjusted via the
software to allow for good mixing of reagents. The optimum injection speed is
dependent on the assay parameters, such as viscosity of fluids, the plate format
and the measuring behavior of the liquids. The removable injector carrier allows
this process to be done outside of the instrument where a visual inspection can
be performed easily.
Before starting a measurement, make sure that:
1. The tubes are clean. If not please refer to chapter 5.6.4 ‘Priming and
Washing of the injector(s)’ for details.
2. The injector tubes are correctly inserted into the storage bottles and
secured.
3. The injector system is primed. It is not possible to start a measurement
without priming the system.
When priming the system:
1. Check the tubes for leaks.
2. Check the tubes for kinks.
3. Make sure that the injector needles are not twisted.
If the tubes require replacement for any reason, after the tubes have been
changed do not forget to perform washing and priming before starting a
measurement.
5.7.4
Priming and Washing of the Injector(s)
STOP
Caution
The injector carrier must be in the service position
for washing und priming. Prime and wash must not be performed when
the injector is in the instrument.
The initial filling step of the injector system (priming) as well as the cleaning step
of the injector system (washing) must take place outside of the instrument. For
these procedures the injector carrier is removed from instrument and put into the
service position of the injector box. For priming and washing steps of the injector
system, a default setting for injection speed and volume dispensed is provided. If
required, the priming parameters can be adjusted in the injector control window of
the i-control software.
The prime volume depends on the tubing length. Two types of injector tubing are
available: ‘long’ = 105 cm (41.34 in.) and ‘short’ = 80 cm (31.5 in.).
The minimal priming volume is 700 µl for an injector with short tubing and 850 µl
for an injector with long tubing.
STOP
Caution
Do not touch the injector needles, as they can become easily bent or
misaligned, which can cause injection problems or damage the
instrument.
