4. Optical System
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
43
4.3.2 Absorbance
Detection
A silicon photodiode is used for the measurement of the transmitted light. It is
sensitive to a wide range of wavelengths. The photodiode is well suited for the
light levels being encountered with absorbance measurements below 4 OD.
Note
For absorbance measurement of nucleic acids in small volumes (2 µl)
use Tecan’s NanoQuant Plate. With this device it is possible to measure
16 different samples in one measurement.
For further information, please contact your local Tecan distributor or
visit: www.tecan.com.
4.4
Luminescence System
For uncompromising performance, the
INFINITE M1000 PRO
has a dedicated
luminescence
detection module
. The luminescence optics have been
designed to meet requirements different from the dedicated fluorescence optics.
The much lower light levels involved when compared to flash lamp induced
fluorescence require the benefits of a photon counting detection technique.
The
INFINITE M1000 PRO
Luminescence System consists of the following parts
(see figure below): luminescence fiber bundle, the filter wheel, and detection unit
(PMT). The luminescence fiber bundle guides the light from the sample through
the filter wheel to the detector. Three different fibers are available for the
INFINITE M1000 PRO
, the fibers are optimized for different plate types: 96, 384,
or 1536-well.
Figure 20: Optical System Luminescence.
