4. Optical System
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
49
Photon Counting Module (PCM)
The PCM, which contains a channel photomultiplier, is designed for applications
in chemo- and bioluminescence. The channel photomultiplier provides a high
dynamic range, which enables luminescence measurements to be made even
with strong variations in light levels. The exceptionally low noise and high
sensitivity allow detection at very low light levels.
4.5.2 AlphaScreen/AlphaLISA
Detection
STOP
Caution
Switch on the instrument at least 15 minutes before starting a
luminescence measurement to ensure stable conditions for the
measurement.
The
INFINITE M1000 PRO
luminescence detection system utilizes the single
photon counting measurement technique. This is based on a dedicated
luminescence PMT with appropriate measurement circuitry. This technique is
very robust against noise and is, therefore, the preferred method for performing
measurements at very low light levels.
Note
AlphaScreen/AlphaLISA measurements are only possible as endpoint
measurements in white or light gray microplates
and cannot be performed in combination
with the injector system or the heating system.
Note
The results of AlphaScreen/AlphaLISA measurements are always
displayed in counts per second, regardless of the integration time used.
4.5.3 AlphaScreen/AlphaLISA Temperature Correction
To compensate for the temperature sensitive nature of AlphaScreen/AlphaLISA
assays, the
INFINITE M1000 PRO
offers a unique temperature correction system
(optional):
A contactless temperature sensor measures the temperature inside each well
and the measured count rates are automatically normalized to a temperature of
22.5 °C.
Note
To ensure the best performance for AlphaScreen/AlphaLISA assays, the
INFINITE M1000 PRO should be operated in a temperature-regulated
environment (±1 °C in the range of 20–25 °C).
