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5. Operating the INFINITE M1000 PRO

62

Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0

2011-09

Time between Move and Flash

When selecting more than one flash per well, a time delay between move and
flash can be set. Due to the stop and go motion of the plate carrier, the meniscus
of the dispensed liquid may vibrate while signal is integrated. This can give rise to
fluctuations of the measured values. The effect has been observed in the wells of
96-well plates and also in larger wells. In particular, it is critical with absorbance
measurements.

5.3.4 FI

Ratio

Mode

Ratio Mode

Up to 5 labels can be measured well-wise. This measurement mode is called
‘ratio mode’. Be aware that no ‘ratio’ calculation is performed after this
measurement. The Excel result sheet shows the raw data. Further calculations
must be performed by the user.

5.3.5 Optimal

Read

(FI

Bottom Measurements Only)

The "Optimal Read" function is available for Fluorescence Bottom
measurements only. The "Optimal Read" function is a measurement on multiple,
spatially-separated spots inside the well. The spots are arrayed to cover the
whole well area in order to achieve maximum well illumination. The total number
of individual measurement spots per well is reflected by the size of the beam
diameter of the Fluorescence Intensity Bottom fiber and is optimized for plate
formats from 12 to 96 wells (see table below).

Plate

Pattern

Number of Spots

1536-well

‘Optimal Read’ option not available

384-well

‘Optimal Read’ option not available

96-well Circle

(filled)

5

48-well Circle

(filled) 21

24-well Circle

(filled) 37

12-well Circle

(filled) 61

6-well

‘Optimal Read’ option not available

“Optimal Read” spot patterns in different plate formats

The flash number per measurement spot is selectable via the software
(1-200 flashes) and the number of measurement spots per well is displayed as
soon as the ‘Optimal Read’ function is activated for a certain plate format in the
fluorescence bottom measurement stripe. The ‘Optimal Read’ function is
available in combination with the 400 Hz flash frequency mode only.
The user-defined ‘total number of flashes’ is automatically distributed over all
measured spots per well. A minor imprecision occurs if an entered flash number
is not divisible without a remainder by the default number of spots for the plate
format used. In this case, the next possible flash distribution that is integrally
divisible by the number of spots per well is calculated, e.g. a measurement with a
total of 26-30 flashes in a 96-well plate (5 single spots) is performed with
6 flashes per spot, whereas a total flash number of 31 results in 7 flashes per
spot.

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Содержание INFINITE M1000 PRO

Страница 1: ...TECAN Instructions for Use for INFINITE M1000 PRO Document Part No 30064852 2011 09 Document Version No 1 0...

Страница 2: ...EUROPE T 43 62 46 89 33 F 43 62 46 72 770 E mail office austria tecan com www tecan com Copyright Information The contents of this document are the property of Tecan Austria GmbH and are not to be cop...

Страница 3: ...rporation WI USA Greiner and Clear and are registered trademarks of Greiner Labortechnik GmbH Frickenhausen Germany HTRF is a registered trademark of Cisbio Bioassays France Hellma is a registered tra...

Страница 4: ...BSERVED WARNING THIS SYMBOL INDICATES THE POSSIBLE PRESENCE OF FLAMMABLE MATERIALS AND A RISK OF FIRE PROPER LABORATORY SAFETY PRECAUTIONS MUST BE OBSERVED ATTENTION DIRECTIVE 2002 96 EC ON WASTE ELEC...

Страница 5: ...TE M1000 PRO No 30064852 Rev No 1 0 5 Symbols Manufactured by Date of manufacture USB label Conformit Europ enne Consult Instructions for Use Directive 2002 96 EC on waste electrical and electronic eq...

Страница 6: ...r Transport Lock 23 3 3 Power Requirements 25 3 4 Switching the Instrument ON 25 3 5 Preparing the INFINITE M1000 PRO for Shipping 27 3 6 Instrument Dimensions 28 3 6 1 INFINITE M1000 PRO Instrument 2...

Страница 7: ...3 6 Measurement Accessories 64 5 4 Optimize Absorbance Measurements 64 5 4 1 Measurement Parameters 64 5 4 2 Absorbance Ratio Mode 65 5 4 3 Measurement Accessories 65 5 5 Optimize Luminescence Measure...

Страница 8: ...1 Detection Limit LOD 96 7 2 2 Uniformity 96 7 2 3 Linearity 96 7 2 4 Accuracy 96 7 2 5 Crosstalk 97 7 2 6 Repeatability Reproducibility 97 7 3 Acceptance Criteria 97 7 4 Test Instructions 98 7 4 1 Fl...

Страница 9: ...We have taken every precaution to ensure that the plate heights and well depths are correct according to the defined plate type These parameters are used to determine the minimum distance between the...

Страница 10: ...laws and guidelines 1 National industrial protection law 2 Accident prevention regulations 3 Safety data sheets of the reagent manufacturers WARNING Depending on the applications parts of the INFINITE...

Страница 11: ...ce spectra In addition to offering absorbance and fluorescence intensity measurements the INFINITE M1000 PRO can also perform fluorescence polarization and luminescence measurements including luminesc...

Страница 12: ...g Hellma 110 QS The cuvette must be inserted horizontally and must be closed tightly to avoid any liquid leakage 2 1 4 Performance The INFINITE M1000 PRO has been designed for speed and sensitivity S...

Страница 13: ...out starting the software The Quick Start Script button is used to start favorite measurement scripts directly from the instrument for further details see the Instructions for Use for the i control so...

Страница 14: ...MB 1 GB Monitor Super VGA Graphics Resolution 1024 x 768 1280 x 1024 Color Depth 256 Mouse Microsoft mouse or compatible pointing device Communication 1 x USB 2 0 2 x USB 2 0 1 x RS232 Serial Devices...

Страница 15: ...a single fluorescent molecule can contribute one fluorescence photon quantum of light This is a part of the energy which has been absorbed before electronic excitation but could not be released fast e...

Страница 16: ...ew York Basel Hong Kong see section 19 Homogeneous Time Resolved Fluorescence Method for Drug Discovery by Alfred J Kolb et al B Fluorescence Time Resolved TRF TRF applies to a class of fluorescent la...

Страница 17: ...citation successfully Using planar polarized light only a specifically oriented subset of the randomly oriented molecules is susceptible to excitation The FP measurement result will be calculated from...

Страница 18: ...Methods in Molecular Biology 102 Humana Press 1998 Flash Type Luminescence with Injectors In flash type luminescence assays the measurement is only performed during the dispensing of the activating re...

Страница 19: ...ce emission optics therefore additional information on the luminescence scan can be found in chapter 4 1 Fluorescence Intensity System and chapter 5 3 Optimize Fluorescence Measurements 2 2 4 AlphaScr...

Страница 20: ...main advantage of Magellan is that data processing capabilities are included In Magellan data is organized and managed as follows Methods can be defined around a test Within Magellan a method includes...

Страница 21: ...t protective Injector dummy mounted Waste tub for plate carrier 3 1 2 Unpacking Instructions Before installing abide by the following instructions 1 Visually inspect the container for damage before it...

Страница 22: ...purposes WARNING The fully equipped INFINITE M1000 PRO is a precision instrument and weighs approximately 29 5 kg At least two people must carefully lift the instrument from the box STOP Caution The...

Страница 23: ...er switch on the back panel of the instrument is in the OFF position 3 Connect the computer to the instrument only with the delivered USB interface cable 4 Insert the power cable into the main power s...

Страница 24: ...remaining screws of the Transport Lock 12 Loosen the two remaining screws and remove the transport lock and confirm the software message by clicking OK 13 The instrument will initialize and is then re...

Страница 25: ...t time after installation it should be left to stand for at least 3 hours so there is no possibility of condensation causing a short circuit Ensure the computer is switched OFF and the instrument s ma...

Страница 26: ...el 7 Main Power Switch 8 Main Power Socket 9 Label Class 1 Laser Product 10 Complies with 21 CFR 1040 10 except for deviations pursuant to Laser Notice No 50 dated June 24 2007 11 Warranty Label 12 Wa...

Страница 27: ...OR USE ARE NOT CORRECTLY PERFORMED THE INSTRUMENT WILL EITHER BE DAMAGED OR THE PROCEDURE WILL NOT BE PERFORMED CORRECTLY AND THE SAFETY OF THE INSTRUMENT CANNOT BE GUARANTEED 3 5 Preparing the INFINI...

Страница 28: ...3 Installation 28 Instructions for Use for INFINITE M1000 PRO No 30064852 Rev No 1 0 2011 09 3 6 Instrument Dimensions 3 6 1 INFINITE M1000 PRO Instrument Front View Figure 6 Side View Figure 7...

Страница 29: ...11 09 Instructions for Use for INFINITE M1000 PRO No 30064852 Rev No 1 0 29 3 6 2 INFINITE M1000 PRO Instrument with Built in Stacker Front View with Built in Stacker Figure 8 Side View with Built in...

Страница 30: ...3 Installation 30 Instructions for Use for INFINITE M1000 PRO No 30064852 Rev No 1 0 2011 09 3 6 3 Injector Module Dimensions Front View Figure 10 Side View Figure 11...

Страница 31: ...rom the light source to and from the top measurement head and to the PMT The path of fluorescence bottom light goes from the light source to and from the bottom measurement head and to the PMT The sys...

Страница 32: ...e c Many applications only one kind of lamp d No warm up time required Condenser Condenser type optics from fused silica focus the flashlight onto the entrance slit of the excitation monochromator Ord...

Страница 33: ...g to 10 3 This means when the monochromator is set for light with a wavelength of 500 nm and the detector detects a signal of 10 000 counts light with different wavelengths creates a signal of only 10...

Страница 34: ...e adjusted As light is refracted onto the sample liquid surface a Z adjustment helps to maximize signal to noise The fluorescence measuring Top and Bottom Optics are built from the following component...

Страница 35: ...on system is used for both measuring modes fluorescence from above top and below the microplate wells bottom The fluorescence light is focused onto the entrance slit of the emission monochromator Afte...

Страница 36: ...a wide range of concentrations in lower or higher concentration domains For details see chapter 5 3 3 Instrument Parameters 4 1 4 Luminescence Scan The INFINITE M1000 PRO is capable of recording emiss...

Страница 37: ...sts of the following parts see figure below LEDs 1 polarization optics 2 emission double monochromator unit 3 and detection unit 4 Figure 14 Optical System Fluorescence Polarization 4 2 1 Light Source...

Страница 38: ...ght so that it is parallel Lens 2 focuses the polarized light into the wells and collects the more or less depolarized emission light from the sample Lens 3 focuses the emission light onto the fiber o...

Страница 39: ...e following components Polarizer Rotator EX Filter 1 4 Dichroic mirror 1 4 Analyzer Figure 16 Fluorescence Polarization Optics Polarizer A polarizer is a device for producing plane polarized light Rot...

Страница 40: ...lyzer allows only light with a specific type of plane to pass 4 2 3 Fluorescence Polarization Detection A fiber bundle guides the polarized light that passes the analyzer to the emission monochromator...

Страница 41: ...r to chapter 4 1 1 Light Source System Fluorescence Intensity A fiber bundle guides the light from the excitation monochromator 2 to the absorbance optics 3 which focus the light into the wells The tr...

Страница 42: ...d to read a 96 well plate The optical alignment for a 384 or 1536 well plate is shown in Figure 19 The software automatically sorts the data and reports it in the correct order Each well is measured w...

Страница 43: ...m 4 4 Luminescence System For uncompromising performance the INFINITE M1000 PRO has a dedicated luminescence detection module The luminescence optics have been designed to meet requirements different...

Страница 44: ...ells thereby minimizing crosstalk Filter Wheel A filter wheel in front of the PMT window is switched to the required luminescence filter channel The sensitivity of the detection system makes it necess...

Страница 45: ...smission spectrum of filter Lumi Magenta Figure 22 Transmission spectrum of filter Lumi Green 0 10 20 30 40 50 60 70 80 90 100 400 420 440 460 480 500 520 540 560 Wavelength nm Transmission Figure 23...

Страница 46: ...rong variations in light levels The exceptionally low noise and high sensitivity allow detection at very low light levels 4 4 2 Luminescence Detection STOP Caution Switch on the instrument at least 15...

Страница 47: ...aScreen AlphaLISA Optics The Z position of the luminescence fiber bundle can be adjusted As light is refracted onto the sample liquid surface a Z adjustment helps to maximize signal to noise and minim...

Страница 48: ...wells thereby minimizing crosstalk Filter Wheel Two additional filters in the luminescence filter wheel allow for measurements of AlphaScreen and AlphaLISA assays The transmission spectra of the Alph...

Страница 49: ...ment circuitry This technique is very robust against noise and is therefore the preferred method for performing measurements at very low light levels Note AlphaScreen AlphaLISA measurements are only p...

Страница 50: ......

Страница 51: ...mize instrument parameters for specific applications Every effort has been made to ensure that the instrument functions correctly even if the default parameters are not appropriate for a particular ap...

Страница 52: ...rument Power On When switching the instrument ON no initialization steps are performed Connect to Instrument When the software connects to the instrument communication is established between the instr...

Страница 53: ...not incubated externally the microplate should be left for equilibration before the measurement is started Kinetic Measurements The i control software allows a plate to be measured repeatedly in equi...

Страница 54: ...p The sensitivity of the reference fiber needs to be adjusted automatically done by the software before each measurement to make sure that the fiber is working in an optimal sensitivity range and does...

Страница 55: ...y a so called G factor The G factor compensates for differences in optical components between parallel and perpendicular measurement The G factor is the correction factor that can be determined for th...

Страница 56: ...tween move and flash of 100ms Calculation of FP Parameters G factor 1 1 par buf par ref ref cross buf cross ref ref RFU RFU P RFU RFU P G Pref Polarization value of reference ref RFU Averaged relative...

Страница 57: ...to digital A D conversion of the PMT signal The gain setting controls the amplification of the PMT when converting fluorescence light into electrical current The A D converter needs a suitable input...

Страница 58: ...ended use of the INFINITE M1000 PRO PMT is specified for gain settings from 60 255 Gain settings below 60 are possible and might be useful for special applications but the performance of the PMT is no...

Страница 59: ...well for further measurements 3 Same as for multi labeling measurements When using the same as option the INFINITE M1000 PRO will automatically use the same z position as for a previously defined lab...

Страница 60: ...and the resulting signal and blank curves are shown in the graph The z position may now be set to the maximum signal to blank S B ratio Figure 31 Note When the option Max S B Ratio is used the sample...

Страница 61: ...re a higher number of single measurement values results in more accurate final measurement values For time resolved fluorescence TRF measurements we recommend using the 100 Hz mode to improve results...

Страница 62: ...pots per well is reflected by the size of the beam diameter of the Fluorescence Intensity Bottom fiber and is optimized for plate formats from 12 to 96 wells see table below Plate Pattern Number of Sp...

Страница 63: ...flashes as well as the number of flashes per well are also displayed Figure 33 Results output for a measurement with optimal read example for a 48 well plate Miscellaneous Features of Optimal Read Op...

Страница 64: ...arameters Flash Settings On the fly measurements with 1 flash per well are possible for all plate types however measurement precision at low light levels depends on the reading time while fluorescence...

Страница 65: ...ssible to measure 16 different samples in one measurement For further information please contact your local Tecan distributor or visit www tecan com 5 5 Optimize Luminescence Measurements 5 5 1 Integr...

Страница 66: ...ion Of course gain value and integration time may be optimized in assays by the user according to the demands of the application Gain optimizations are also necessary if temperature dependent experime...

Страница 67: ...he average rate of which can be measured using a counter The advantage of using the photon counting technique at such low light levels is that pulse height selection criteria allow much of the electro...

Страница 68: ...the injector module The INFINITE M1000 PRO can be equipped with one pump or two pumps Figure 34 Pump A feeds injector needle A Pump B feeds injector needle B One Injector Option one pump An INFINITE...

Страница 69: ...ing measurement modes Fluorescence Intensity top and bottom Time Resolved Fluorescence Absorbance Flash and Glow Type Luminescence and Dual color Luminescence However as the measurement position is no...

Страница 70: ...one 15 ml bottle Two Injectors option one 125 ml bottle and two 15 ml bottles The injector option includes up to two bottle holders that are designed for tubes of different sizes and volumes The bottl...

Страница 71: ...oning of the injector carrier in the instrument If the injector is not inserted correctly the injector sensor will not recognize the inserted carrier and neither dispensing nor injection will be possi...

Страница 72: ...injector needles are not twisted If the tubes require replacement for any reason after the tubes have been changed do not forget to perform washing and priming before starting a measurement 5 7 4 Pri...

Страница 73: ...performance 7 Visually inspect the tube s for leaks and kinks 8 Check the liquid jet during priming Prime 1 Select one of the injectors Injector A or Injector B or Injector A and B 2 Select the Prime...

Страница 74: ...ect the Backflush Speed 100 300 l sec 4 Select the Refill Speed 100 300 l sec or select Refill Speed equal to Backflush Speed check box 5 Press Start Backflush to start the reagent backflush procedure...

Страница 75: ...onds 7 Select Close to exit the dialog box STOP Caution The injector carrier must be in the service position for the action wash Do not perform wash when the injector is in the instrument STOP Importa...

Страница 76: ...injection the dispense step is only performed once when starting the measurement The waste tub must therefore be emptied from time to time The maximum filling volume is 2 ml The internal counter check...

Страница 77: ...igure 38 Dispense Select Injector Injector A and or Injector B can be selected Speed The injection speed is selectable from 100 300 l sec for each injector Select Refill speed from 100 300 l sec for e...

Страница 78: ...h injector A and afterwards with injector B The dispense strip does not require an additional measurement strip Dispense volume The dispense volume depends on the microplate type The plate definition...

Страница 79: ...with two injectors is to be performed two injector strips are necessary Speed The injection speed is selectable from 100 300 l sec for each injector Select a Refill speed from 100 300 l sec for each i...

Страница 80: ...microplate type The plate definition files include the working volume of the microplate This working volume defines the maximum volume to be dispensed into the selected microplate Therefore ensure th...

Страница 81: ...stem Daily Maintenance If not otherwise stated by the manufacturer of the kit to be used at least the following tasks must be performed daily Inspect the pump s and tubing for leaks Flush the whole sy...

Страница 82: ...OH 4 Set up a software script consisting of a Plate stripe with a plate definition file corresponding to the inserted type of microplate Part of the Plate Well A1 and a Dispense stripe 5 Select inject...

Страница 83: ...lene Diamine Hexane Furfural Methanol Methyl Alcohol Hydrofluoric Acid Sulfuric Acid diluted Concentration 1 N Monoethanolamine Tetrahydrofuran Potassium Hydroxide Caustic Potash Water Deionized Potas...

Страница 84: ...eight is 23 mm The INFINITE M1000 PRO may be optionally equipped with a built in stacker for loading and un loading standard microplates automatically Figure 42 Only plates without lids can be execute...

Страница 85: ...eft or right side of the plate transport INFINITE M1000 PRO is a LASER CLASS 1 product INFINITE M1000 PRO complies with FDA radiation performance standards 21 CFR 1040 10 except for deviations pursuan...

Страница 86: ...r the instrument is a class I laser device The following label is attached to the rear of the instrument The production date of the barcode scanner may be found on the rear of the barcode reader insid...

Страница 87: ...RF TR FRET Fluorescence Polarization Absorbance Glow Type Luminescence Flash Type Luminescence Dual color Luminescence AlphaScreen AlphaLISA Injector option for Fluorescence Absorbance Luminescence Al...

Страница 88: ...Parameters Characteristics Light source High energy Xenon flash lamp Optics Fused Silica Lenses Fluorescence Detector Low dark current photomultiplier tube Luminescence Detector Low dark count photom...

Страница 89: ...om Well automatic 0 60 000 RFU TRF Parameters Characteristics Integration Time 20 2000 s Lag Time 0 2000 s 6 3 1 Definition of the Detection Limit The detection limit is the fluorophore concentration...

Страница 90: ...n Detection Limit pM 35 40 6 3 4 Europium Time Resolved Fluorescence Plate type number of wells 384 1536 Dispensed Volume l 100 10 Flashes per Well 12 12 Flash frequency 100 Hz 100 Hz Europium Detecti...

Страница 91: ...Fluorescence TRF 6 4 1 Fluorescein 1nM Fluorescence Polarization Plate type number of wells 384 1536 Dispensed Volume l 100 10 Flashes per Well 1 flash 10 ms integration time 10 10 Fluorescein 1 nM P...

Страница 92: ...ell 0 035 ATP Integration Time well 100 20000 ms Attenuation No attenuation Automatic OD2 6 6 1 ATP Glow Luminescence Plate type number of wells 384 1536 Total Dispensed Volume l 100 10 Integration Ti...

Страница 93: ...fferent integration times are possible for each wavelength Plate Type 96 384 and 1536 well microplates Dynamic Range 6 decades 6 7 AlphaScreen AlphaLISA STOP Caution Switch on the instrument at least...

Страница 94: ...asurement Time 96 well 384 well 1536 well Absorbance 11 s 15 s 23 s Fluorescence Intensity Top 17 s 24 s 36 s Fluorescence Intensity Bottom 19 s 25 s 38 s 6 9 Injectors Parameters Characteristics Plat...

Страница 95: ...structions carefully The user should determine the appropriate intervals for this testing based on how frequently the instrument is operated We recommend adapting these tests and the acceptance criter...

Страница 96: ...well to well variations when measuring a multi well plate The uniformity is either calculated as a percentage deviation from the mean value or for low OD values as a maximal deviation in mOD 7 2 3 Li...

Страница 97: ...approvals The following table provides an overview of the passed failed criteria for the specification tests of the INFINITE M1000 PRO Specification Acceptance Criteria Plate type number of wells 384...

Страница 98: ...te black flat bottom according to the plate layout Material Greiner 384 well plate flat bottom black 100 l pipette 8 channel tips 1 nM Fluorescein diluted in 0 01 M NaOH 0 01 M NaOH Plate Layout 1 2 3...

Страница 99: ...ean mean 1000 imit DetectionL meanF average of wells filled with 1 nM Fluorescein meanB average of wells filled with Blank stdevF standard deviation of wells with Blank 7 4 1 2 Uniformity Top Fluoresc...

Страница 100: ...NaOH 0 01 M NaOH Plate layout 1 5 9 13 17 21 25 29 33 37 41 45 A F B F B F B F B F B F B E F B F B F B F B F B F B I F B F B F B F B F B F B M F B F B F B F B F B F B Q F B F B F B F B F B F B U F B...

Страница 101: ...6 well Plate Pipette 10 l of the 1 nM Fluorescein solution into the wells of a Greiner 1536 well plate black flat bottom as described in chapter 7 4 1 1 Detection Limit Top Fluorescein 384 well Plate...

Страница 102: ...01 M NaOH Plate Layout 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A F B F B F B F B F B F B B C F B F B F B F B F B F B D E F B F B F B F B F B F B F G F B F B F B F B F B F B H I...

Страница 103: ...ll Plate Pipette 100 l of the 1 nM Fluorescein solution into the wells of a Greiner 384 well plate black Clear flat bottom as described in chapter 7 4 1 5 Detection Limit Bottom Fluorescein 384 well P...

Страница 104: ...F B F B F B F B F B F B M F B F B F B F B F B F B Q F B F B F B F B F B F B U F B F B F B F B F B F B Y F B F B F B F B F B F B AC F B F B F B F B F B F B AE F 10 l 1 nM Fluorescein B 10 l 0 01 M NaOH...

Страница 105: ...flat bottom as described in chapter 7 4 1 7 Detection Limit Bottom Fluorescein 1536 well Plate Material See chapter 7 4 1 7 Detection Limit Bottom Fluorescein 1536 well Plate 0 01 M NaOH Measurement...

Страница 106: ...B B F G B B B B B B B B B B B B H I B B B B B B B B B B B B J K B B B B B B B B B B B B L M B B B B B B B B B B B B N O B B B B B B B B B B B B P Eu 100 l 1 nM Europium B 100 l Enhancement Solution Bl...

Страница 107: ...B B B B B B C Eu B B B B B B B B B B B B B B B B D E I M Q U Y AC Eu 10 l 1 nM Europium B 10 l Enhancement Solution Blank Measurement Parameters Measurement mode Fluorescence Top Excitation wavelengt...

Страница 108: ...F B F B F B F B F B H I F B F B F B F B F B F B J K F B F B F B F B F B F B L M F B F B F B F B F B F B N O F B F B F B F B F B F B P F 100 l 1 nM Fluorescein B 100 l 0 01 M NaOH Blank P 100 l Invitr...

Страница 109: ...B F B F B F B F B F B U F B F B F B F B F B F B Y F B F B F B F B F B F B AC F B F B F B F B F B F B F 10 l 1 nM Fluorescein B 10 l 0 01 M NaOH Blank P 10 l Invitrogen HighPolStandard Measurement Par...

Страница 110: ...P Bx B B B B B B B C Bx ATP Bx B B B B B B B D Bx ATP Bx B B B B B B B E F G H ATP 200 l 2 10 8 M ATP B 200 l Blank Bx 200 l Blank used for crosstalk check Measurement Parameters Before pipetting the...

Страница 111: ...24 A Bx ATP Bx B B B B B B B B Bx ATP Bx B B B B B B B C Bx ATP Bx B B B B B B B D Bx ATP Bx B B B B B B B E F G H I J K L M N O P ATP 100 l 2 10 8 M ATP B 100 l Blank Bx 100 l Blank used for crossta...

Страница 112: ...ent results of chapter 7 4 4 2 Detection Limit ATP 384 well Plate for each well separately The following wells are charged together A2 with A1 A2 with A3 B2 with B1 B2 with B3 C2 with C1 C2 with C3 D2...

Страница 113: ...ls A2 D2 add 8 l of ATP reagent and mix in well use fresh tip for each well ATP reagent must NOT be contaminated with ATP standard 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 25 33 41 A Bx ATP Bx B B B...

Страница 114: ...ther A2 with A1 A2 with A3 B2 with B1 B2 with B3 C2 with C1 C2 with C3 D2 with D1 and D2 with D3 The average of these 8 values must be within the specified limits see chapter 7 3 Acceptance Criteria u...

Страница 115: ...Before pipetting the plate prepare instrument for measurement Parameters Measurement mode AlphaScreen AlphaLISA Excitation time 100 ms Integration time 300 ms Temperature correction activated Plate d...

Страница 116: ...adding 3 l of the stock solution 5 mg ml to 5997 l PBS yielding a solution of 2 5 g ml Plate layout Pipette 100 l of the Omnibeads dilution the highlighted wells of the microplate as shown in the plat...

Страница 117: ...ner 384 well plate flat bottom white Phosphate buffered saline PBS 100 l pipette tips Omnibeads dilution series Dilute the Omnibeads stock solution 1 2000 in PBS by adding 0 5 l of the stock solution...

Страница 118: ...Evaluation Calculate the crosstalk B OB 100 B Bx Crosstalk OB signal of well filled with Omnibeads B average signal of blank wells B Bx average signal of wells surrounding the Omnibeads well Bx 7 4 6...

Страница 119: ...24 A OG OG OG OG OG OG OG OG OG OG OG OG B C OG OG OG OG OG OG OG OG OG OG OG OG D E OG OG OG OG OG OG OG OG OG OG OG OG F G OG OG OG OG OG OG OG OG OG OG OG OG H I OG OG OG OG OG OG OG OG OG OG OG O...

Страница 120: ...tilled water Blank 100 l pipette tips Plate layout 100 l well Measurement Parameters OG Measurement mode Absorbance Measurement wavelength 492 nm Number of flashes 50 Plate definition file GRE384ft pd...

Страница 121: ...ecision Pr Average Average of well over 20 cycles Measured Measured value of one well one time point mean 100 stdev CV Precision mean average of well over 20 cycles stdev standard deviation of well ov...

Страница 122: ......

Страница 123: ...reduce the need for servicing STOP Caution Keep the plate transport clean especially take care of the clip mechanism to fix microplates Insufficient plate fixing lead to instrument damages Strong soi...

Страница 124: ...for shipment If a microplate is left in the instrument fluorescent solutions may spill onto the optical parts and damage the instrument Before the instrument is returned to the distributor or service...

Страница 125: ...is on the plate carrier remove it 4 Disconnect the instrument from the main power supply and let it cool down to ambient temperature to avoid any risk of fire and explosion 5 Disconnect the instrumen...

Страница 126: ...lete two copies of the Safety Certificate which was delivered with the instrument and attach one copy to the top of the container in which the instrument is returned visible from the outside of the sh...

Страница 127: ...electrical and electronic equipment WEEE Negative environmental impacts associated with the treatment of waste z Do not treat electrical and electronic equipment as unsorted municipal waste z Collect...

Страница 128: ...IATE COLLECTING POINTS AND APPROVED METHODS OF DISPOSAL IN YOUR COUNTRY STATE OR REGION 8 4 3 Disposal of the Instrument Please contact your local Tecan service representative before disposing of the...

Страница 129: ...Please report this error to your local Tecan customer support office ERR 5 Invalid Parameter at pos Unspecific error in the Instrument Computer communication protocol Please report this error to your...

Страница 130: ...Please report this error to your local Tecan customer support office ERR 35 Invalid Parameter Length max n char allowed Unspecific error in the Instrument Computer communication protocol Please report...

Страница 131: ...eport this error to your local Tecan customer support office ERR 74 Checksum Error Excitation Checksum error on excitation data channel Please report this error to your local Tecan customer support of...

Страница 132: ...132 Instructions for Use for INFINITE M1000 PRO No 30064852 Rev No 1 0 2011 09 Error Error Text Description ERR 86 ST MEAS HW Error 10 99 Emission or excitation busy Please report this error to your...

Страница 133: ...iber Bundle 34 Fluorescence Intensity Detection 35 Fluorescence Intensity Lens System 34 Fluorescence Intensity System 31 Fluorescence Polarization 17 91 Fluorescence Polarization Detection 40 Fluores...

Страница 134: ...57 P Packing Material Disposal 127 Returning 127 Performance 12 Plate Carrier Transport Lock 23 PMT Detector 36 Polarizer 39 Power Requirements 25 Q Quality Control 95 R Reagent Compatibility 83 S Saf...

Страница 135: ...Spain N VI km 23 300 c Pollensa 4 Oficina 8 E 28230 Las Rozas de Madrid Spain T 34 91 151 71 07 F 34 91 151 7120 helpdesk sp tecan com Tecan Ib rica Portugal Quinta da Fonte Edificio Pedro I P 2780 7...

Страница 136: ......

Страница 137: ...in consideration EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements EN 61010 2 081 A1 2003 Safety requirements for ele...

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