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Tip!
If the pane with the TWC is closed, then click a point anywhere in the TWC to open it again. Click
Explore > Show > Show DAD TWC.
Generate TWCs
A TWC is a less commonly used chromatogram. It shows the total absorbance (mAU) as a function of time. The
TWC provides a way of viewing an entire data set in a single pane. It consists of the summed absorbances of all
ions in a scan plotted against time in a chromatographic pane. If the data contains results from multiple experiments,
then create individual TWCs for each experiment and another TWC that represents the sum of all experiments.
A TWC shows total absorbance (mAU) on the Y-axis plotted against time on the X-axis. Refer to
for more information about using the available icons.
1. Open a data file that contains a DAD spectrum.
2. Click
Explore > Show > Show DAD TWC.
The TWC is shown in a pane below the DAD spectrum.
Tip!
Right-click inside the pane containing the DAD spectrum and then click
Show DAD TWC
.
Adjust the Threshold
The threshold is an invisible line drawn parallel to the X-axis of a graph that sets a limit below which the software
will not include peaks in a spectrum. The line has a handle, represented by a blue triangle to the left of the Y-axis.
Click the blue triangle to view a dotted line that represents the threshold. The threshold can be raised or lowered,
but changing the threshold value does not change the data. The software does not label any peaks in the region
that lies below the threshold.
1. Open a data file.
2. Do one of the following:
• To raise the threshold, drag the blue triangle up the Y-axis. To lower the threshold, drag the blue triangle
down.
• Click
Explore > Set Threshold.
In the Threshold Options dialog that opens, type the threshold value
and then click
OK
.
• Click
Explore > Threshold.
The graph updates to show the new threshold. Peak labeling and the peak list are also updated.
Tip!
To view the current threshold value, move the pointer over the threshold handle.
TripleTOF
®
5600/5600+ System
System User Guide
87 / 131
RUO-IDV-05-7040-B
Operating Instructions — Analyze and Explore Data