Olympus FLUOVIEW FV300, Руководство пользователя

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Getting Started FLUOVIEW/
Outline of LSM Observation Procedures
IV.
OPERATION INSTRUCTIONS
IV
. 1 - 5 0
Page
1-2-12-10 Stopping Repeated Scanning
After the brightness and gain have been adjusted, select the <STOP SCAN> button in
the [Acquire] panel to stop scanning temporarily.
1-2-13 Adjusting the Detection Light Axis
Adjust the detection light axis for efficient acquisition of the detection light.
This adjustment is particularly important after the excitation DM has been switched using
the excitation DM selector knob because this operation tends to deviate the light axis.
1. Select the <XY Repeat> button in the [Acquire] panel. The image appears in the
[Live] panel.
2. If no image appears in the [Live] panel, increase the confocal aperture number by 1
using the confocal aperture rotary knob (1).
3. While observing the image in the [Live] panel, adjust the detection light axis
adjustment knobs on the top and front of the scan unit to maximize the brightness
of the image in the [Live] panel.
4. Decrease the confocal aperture number by 1 using the confocal aperture rotary
knob (1).
5. Repeat steps 1 to 4 until the optimum confocal aperture number is set. For the
confocal aperture number recommended for each objective, see section 1-1-1.
If the detection light axis adjustment is difficult due to dark image,
adjust the PMT Voltage as described in section 2-2-1-3-9.
1-2-14 Acquiring Image
Select the <Once> button. The acquired image will be displayed in the [Live] panel.
<XY Repeat> button
<Once> button
NOTE