Olympus FLUOVIEW FV300, Руководство пользователя

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SYSTEM OVERVIEW/
Principle
III .
SYSTEM OVERVIEW
III .
1 - 1
Page
1 SYSTEM OVERVIEW
Olympus FLUOVIEW is a confocal scanning type laser reflected fluorescence microscope with high resolution,
high contrast and drastically improved resolution in the light axis direction thanks to the use of confocal optics.
It offers researchers features for sectioning, 3D construction and time-series observation as well as a variety of
image processing and analysis functions.
This section describes the principle and characteristics of the system and the functional configuration of the
software used with the system.
1-1 Principle
A laser-scanning microscope concentrates a laser beam on a very small spot using an
objective and scans the specimen in the X-Y directions.
It then detects the fluorescence and transmitted light from the specimen using light
detector and outputs the image of the
specimen on an image monitor.
The confocal optics place a confocal
aperture at a position which is optically
conjugate with the focusing position (i.e.
confocal plane) in order to eliminate
light from parts other than those for the
focusing position. As a result, the part
corresponding to the eliminated light is
darkened in the image, making it
possible to optically slice a thick tissue
specimen.
On the other hand, with ordinary optical
microscopes, light from a part other
than the focusing position are
overlapped from the image-forming
light on the focusing position, and the
overall image tends to be vague.
Transmitted light images can be
obtained by use of a transmitted light
detector, but the images will not be
confocal image, because there is no
confocal aperture in front of the detector.
The transmitted light image can be used by combination with fluorescence image to
observe fluorescent localization.
Confocal aperture
Light detector
Objective
Specimen
Light detector
Laser