PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution
Assays/Reagents
AEROSET
®
System Troubleshooting Guide
125
94816-107—November 2004
Creatine Kinase
FlexRate™ linearity is not stated in the Linearity section of the
CK package insert; however, Flex Read times (22 – 25) and Abs
Limits (0 – 2.8) are configured in the assay parameters.
The Flex Read Time is used if less than three data points fall
within the absorbance range during the Main Read Time.
However, because of the nature of the CK assay, this condition
does not occur, and the Flex Read Time is never utilized by the
AEROSET System for the CK assay. Linearity of CK is
4,267 U/L. To obtain a higher value than 4, 267, the result can
be auto diluted 1:2 or 1:10 using the dilution protocols defined
for the CK assay.
Digoxin (MULTIGENT
®
)
Spironolactone/Canrenone Interference Results
A case report by Steimer et al has shown that a negative bias in
the determination of digoxin results may be observed when
the aldosterone inhibitor spironolactone or canrenone is
present in serum. A sample containing 2.0 ng/mL of digoxin
was spiked separately with 800 µg/L spironolactone and
3,000 µg/L canrenone and analyzed with the MULTIGENT
Digoxin assay. The following results demonstrate no
significant interference.
Reference: Steimer W, Muller C, Eber B, et al. Intoxication due
to negative canrenone interference in digoxin drug
monitoring.
Lancet
1999;354:1176–7.
Sample
Control
Concentration
Recovered
Compound
(ng/mL
Digoxin)
(nmol/L
Digoxin)
(ng/mL
Digoxin)
(nmol/L
Digoxin)
Spironolactone
1.98
2.53
1.98
2.53
Canrenone
2.07
2.65
2.12
2.71
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