Zeiss Lightsheet Z.1, Operating Manual

Page: 42 / 427

CHAPTER 1 - HARDWARE
Carl Zeiss User Interfaces Lightsheet Z.1
38 000000-1790-528 02/2013
Make sure that the temperature inside the sample chamber is in accordance with the desired
temperature value required for the experiment. The ZEN software displays the temperature of the
heating block itself and does not register the actual temperature in the sample chamber that can
be lower (due thermal loss) or higher.
•
To assemble the heating block to the sample chamber remove first of all the sample chamber dove tail
slide from the assembled sample chamber (Fig. 1, section
3.3.2 Assembly of the Sample Chamber
Body and the Sample Chamber Dove Tail Slide
).
•
Take the heating block and screw it onto the bottom of sample chamber body following the
instructions of the individual component manuals from PeCon.
•
The sample chamber body can now be assembled together with the sample chamber dove tail slide
(Fig. 1, section
3.3.2
).
•
Insert the sample chamber as described in chapter 2.4 and plug in the 8 pin connector of the
connection cable into the right side of the front system cavity / sample room following the individual
component manuals from PeCon (Fig. 32/
6 ) shows the connection exemplary for the Peltier Block S).
(2) Peltier Block S is part of the TempModule CZ-LSFM. The Peltier Block helps adjust the sample
chamber temperature by heating or cooling the sample chamber within the range of 10 °C to 42 °C (up
to 1.5°/min heating, up to 1.0°/min cooling). The temperature inside the sample chamber is monitored by
a temperature sensor (Fig. 23/
1
; Fig. 32/
3
). For insertion of the temperature sensor in the sample
chamber refer to section 3.3.4 Insertion of Accessories for Incubation ).
The TempModule CZ-LSFM contains a water based coolant reservoir. For safety reasons (Risk of
electrical shock as a result of leakage) the unit must be placed directly on a level table and is thus
the base for all other incubation related modules (Fig. 31).
−
Connect the TempModule S, the TempModule CZ-LSFM, the Lightsheet Z.1 and the Peltier Block
according to the individual component manuals from PeCon.
−
To assemble the Peltier Block to the sample chamber remove first of all the sample chamber dove
tail slide from the assembled sample chamber (section
3.3.2 ; Fig. 21).
−
Take the Peltier Block and screw it onto the bottom of sample chamber body following the
instructions of the individual component manuals from PeCon.
−
The sample chamber body can now be assembled together with the sample chamber dove tail slide
(Fig. 21) and insert it into the Lightsheet Z.1 as described (section
3.3.2 ).
−
Insert the accessories for incubation (temperature sensor, cover recommended) into the sample
chamber (section
3.3.4 , Fig. 23, Fig. 24).
−
Connect the Peltier Block (Fig. 32/ 6 ) to the Lightsheet Z.1 by the two provided silicone tubes
(Fig. 32/
7-8
) and plug in the 8 pin connector of the connection cable into the right side of the front
system cavity / sample room (Fig. 32). For proper electrical connections and tube connections follow
the instructions of the PeCon manual.