Carl Zeiss
Left Tool Area and Hardware Control Tools
Lightsheet Z.1
102
000000-1790-528
02/2013
3.3.8.2
Ratiometric Dyes
•
Fura-2, Indo-, SNARF, Cameleon, Ratiometric Pericam, Phluorin.
•
Display fluorescence ratio R over time
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Display fluorescence ratio R corrected for background/autofluorescence over time
•
Calculate absolute ion concentrations (pixel by pixel) via titration calibration (known ion concentrations
applied to the cells – in situ – or in solutions – in vitro or equation calibration where possible [Fura-2,
Indo-, SNARF]
•
Calculation of R eliminates artifacts and uncertainties caused by
−
inhomogeneous dye distribution
−
photobleaching
−
may be applied with moving cells.
Ratiometric Dyes - Online Ratio
R(t1) = F1(t1) / F2(t1), R(t2) = F1(t2) / F2(t2) ..
Fig. 149
Ratiometric Dyes – online ratio
Summary of Contents for Lightsheet Z.1
Page 1: ...Lightsheet Z 1 Operating Manual February 2013 ZEN 2012 black edition ...
Page 4: ......
Page 170: ......
Page 427: ...Lightsheet Z 1 Overview ...