Zeiss Lightsheet Z.1, Operating Manual

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CHAPTER 4 - SYSTEM OPERATION
Carl Zeiss Left Tool Area and Hardware Control Tools Lightsheet Z.1
108 000000-1790-528 02/2013
The
Sampling
mode is a meant to improve the image display as it equalizes the differences between the
very high and the very low intensities in the image. However, it is not suitable for quantitative analysis as
the level steps become non-linear.
The image re-calculation works already with a single image (
Frame
= 1) and is done in the following way
taking Intensity 3.
Image x 1; cut off of grey levels above the dynamic range; Result divided by 1
Image x 2; cut off of grey levels above the dynamic range; Result divided by 2
Image x 3; cut off of grey levels above the dynamic range; Result divided by 3
The three resulting images are summed up and again divided by 3. This procedure is adapted accordingly
if a higher
Intensity number is set. If two images are acquired ( Frame = 2) and Intensity is still at 3, the
second image is rated lower and is multiplied and divided in three steps with the value 4,5 and 6; The
then 6 resulting images are summed up and divided by 6.
The image acquisition parameters are not changed for the acquisition of the two (or more)
Frames .
Fig. 157 HDR image display (left side without, right with HDR)