Zeiss Lightsheet Z.1, Operating Manual

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CHAPTER 4 - SYSTEM OPERATION
Lightsheet Z.1 Left Tool Area and Hardware Control Tools Carl Zeiss
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All processing tools can be used with stored and reloaded images or with newly acquired images
(that need not be stored before processing).
Please note that for PALM images, although stored in a .czi format, only the PALM and Channel
Alignment Processing tools are available, which can recognize tables. If you want to apply other
Processing tools, the PALM image based on the table has to be converted to a true image. Such a
function is available as a sub-function in the PALM Processing tool.
Calculated high resolution PALM images are stored as .czi format, however they are based on a
table and only few functions can be used with those images. If you want to have access to other
functions, PALM images have to be first converted to true images. Calculated SIM images, on the
other hand, have the same format as normal wide field images.
Note that all .lsm and .czi images can be loaded into a function, but in order for that function to
make sense, the .lsm or .czi image format must have the right dimensions.