Zeiss Lightsheet Z.1, Operating Manual

Page: 327 / 427

CHAPTER 4 - SYSTEM OPERATION
Lightsheet Z.1 Left Tool Area and Hardware Control Tools Carl Zeiss
02/2013 000000-1790-528 157
3.3.19.2 ICS Correlation
•
Activate
ICS correlation
in the
ICS
tool. The
ICS correlation menu will appear (Fig. 244).
•
Highlight the image in the image container and
press the
Select button. This will be your Input
image.
•
This function is only available in the LSM
imaging mode
A correlation image will be computed based on the
settings and displayed in the
Preview window. As
an output image an image with the selected
average subtracted is computed and the result
presented in the
Preview
window.
•
The correlation to be computed can be selected
from the
Correlation drop down menu
(Fig. 245). For one channel recordings, only
Spatial auto-correlation (S+A)
is available, for
a two channel recording in addition Spatial
cross-correlation (S+C)
.
The way of average subtraction can be selected
from the
Remove structures
drop down menu
(Fig. 246).
•
You can select between None (no average
subtraction),
Slowly moving structures
(subtraction of moving average, the frame size
of which can be selected in the
Average
frames input box), Immobile structures
(+Stack)
and
Immobile structures (+Plane)
(subtraction of total average and adding
average scalar of all pixels from one frame to
each corresponding frame).
Fig. 244 Processing – ICS correlation
Fig. 245 Correlation drop down menu
Fig. 246 Remove structures drop down menu