Zeiss Lightsheet Z.1, Operating Manual

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CHAPTER 4 - SYSTEM OPERATION
Lightsheet Z.1 Left Tool Area and Hardware Control Tools Carl Zeiss
02/2013 000000-1790-528 41
To have access to the respective controls, expand
the fields by pressing the triangles (Fig. 44).
The
Temperature (
o
C) panel allows you to control
up to 2 independent heating channels that are
linked to certain devices (Peltierblock Sample
Chamber Lightsheet Z.1, Heatingblock Sample
Chamber Lightsheet Z.1) that are assigned to
different channels. Enter the desired nominal
temperature via the input boxes by typing in the
numbers or by using the arrows to set the
parameters. Check the corresponding box to
activate the desired
Channel . The grey numbers
on the right will display the actual temperature.
The
Atmosphere (%)
panel allows defining CO
2
concentration via the input boxes. Either type in
the numbers or use the arrows to set the
parameters. Actual values will be displayed on the
right.
3.2 Acquisition Tab
If Acquisition is selected, the Acquisition panel
becomes available (Fig. 45).
Acquisition contains all functions of the system
that are needed to set up and control light sheet
fluorescent imaging and multidimensional data
acquisition.
Depending on the hardware configuration
determines which of the described functions are
available.
For excitation, all imaging modes use lasers. The
Acquisition
panel is divided into the
−
Experiment Manager
field for loading
predefined configurations, action buttons
and definition of the multidimensionality of
the experiment and
−
Tool Group
field with the
tool tabs
for
setting the imaging parameters.
Fig. 44 Incubator panel – Expanded fields for
atmosphere and temperature control
Fig. 45 Acquisition panel – appearance