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Olympus BX51, Operating Manual

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Olympus BX51 Operating Manual

http://www.manuallib.com/file/2605418

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This Manual: http://www.manuallib.com/file/2605418

Summary of Contents for BX51

Page 1: ...b com ManualLib com collects and classifies the global product instrunction manuals to help users access anytime and anywhere helping users make better use of products Home http www manuallib com Chin...

Page 2: ...3 Phase Contrast 5 Nomarski Differential Interference Contrast DIC 7 Simultaneous Fluorescence and Phase Contrast 9 Simultaneous Fluorescence and DIC 11 Polarized Light 13 APPENDIX 1 Outline Olympus B...

Page 3: ...ve by rotating the nosepiece 12 and focus Continue until you reach the desired magnification 12 Establish Koehler illumination Close field iris diaphragm 13 until you can see the edges Focus the image...

Page 4: ...hen finished Lower the stage by turning the focus knob 10 towards you and remove the slide with your specimen from the stage 7 Turn the nosepiece 12 back until the 10x objective is into place If the10...

Page 5: ...ris diaphragm knob 22 the aperture iris diaphragm will get smaller 9 Examine specimen and document image if necessary If using 35mm film use checklist for Olympus 35mm Camera To obtain still digitized...

Page 6: ...U 1 Autofluorescence observation DAPI DNA staining Hoechst 33258 33342 Chromosome WB 2 FITC Fluorescent antibody Acridine orange DNA RNA Auramine Tubercle bacillus EGFP S65T RSGFP WG 3 Rhodamine TRITC...

Page 7: ...13 until you can see the edges Focus the image of the field iris diaphragm by raising or lowering the condenser using the condenser height adjustment knob 14 Check if the circle of light is centered i...

Page 8: ...emove the slide with your specimen from the stage 7 Turn the nosepiece 12 back until the 10x objective for brightfield imaging is into place Turn the light intensity down using the brightness adjustme...

Page 9: ...u can see the edges Focus the image of the field iris diaphragm by raising or lowering the condenser using the condenser height adjustment knob 14 Check if the circle of light is centered in the field...

Page 10: ...the polarizer 25 out of the light path Pull the reflected light analyser U AN 24 out of the light path Turn the light intensity down using the brightness adjustment knob 3 Turn off the halogen light...

Page 11: ...you the observed image and to improve the contrast pull out the aperture iris diaphragm knob 22 the aperture iris diaphragm will get smaller 9 Examine specimen and document image if necessary If usin...

Page 12: ...ff the halogen light switch 1 to 0 OFF Cover the microscope when the mercury bulb is cooled down 15 minutes after it was turned off Leave the room CLEAN and TIDY This Manual http www manuallib com fil...

Page 13: ...of view 8 To help adjusting the brightness of you the observed image and to improve the contrast pull out the aperture iris diaphragm knob 22 the aperture iris diaphragm will get smaller 9 Examine sp...

Page 14: ...path Pull the polarizer 25 out of the light path Pull the reflected light analyser U AN 24 out of the light path Turn the light intensity down using the brightness adjustment knob 3 Turn off the halog...

Page 15: ...light on the specimen Close field iris diaphragm 13 until you can see the edges Focus the image of the field iris diaphragm by raising or lowering the condenser using the condenser height adjustment k...

Page 16: ...x objective was used clean the lens carefully with lens paper ONLY do not use Kimwhipes Pull the polarizer 25 out of the light path Pull the reflected light analyser U AN 24 out of the light path Turn...

Page 17: ...15 APPENDIX 1 Outline Olympus BX51 Microscope This Manual http www manuallib com file 2605418...

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