1
Operating Manual for the Olympus BX51
BRIGHTFIELD
1.
Remove the microscope cover.
2.
Turn on the halogen light switch (
1
) for transmitted light to “I” (ON).
3.
Check the light path. The light path selector knob (
2
) should be pushed in all the way.
4.
Adjust the light intensity using the brightness adjustment knob (
3
). The numerals to the right
of the lamp voltage indicator LEDs (
4
) indicate the voltage.
5.
Adjust the interpupillar distance. While looking through the eyepieces, adjust oculars (
5
)
until the left and right fields of view coincide completely.
6.
Make sure the 10x objective (
6
) is in place.
7.
Place the slide with your specimen on the stage (
7
) and hold it with the specimen holder (
8
).
8.
Find your specimen using the stage controls (
9
).
9.
Focus specimen using fine/course focussing knobs (
10
).
10.
Adjust the diopter:
-
Close your left eye and focus on the specimen using the fine focus knob.
-
Close your right eye and focus on the specimen using the diopter ring (
11
) on the left
ocular.
-
Open both eyes and confirm that the focus is comfortable.
11.
If desired switch to the next objective by rotating the nosepiece (
12
) and focus. Continue
until you reach the desired magnification.
12.
Establish Koehler illumination:
-
Close field iris diaphragm (
13
) until you can see the edges.
-
Focus the image of the field iris diaphragm by raising or lowering the condenser
using the condenser height adjustment knob (
14
).
-
Check if the circle of light is centered in the field of view. If not, use the two
condenser centering screws (
15
) to move the field iris diaphragm image to the center
of the field of view.
-
Open the field iris diaphragm until its image circumscribes the field of view.
-
Match the opening of the condenser aperture iris diaphragm (
16
) with the N.A. of the
objective in use in order to achieve the optimum objective performance.
NOTE:
Most specimen are usually low in contrast, reducing the diaphragm opening
to 70%-80% of the N.A. value of the respective objective will generally provide an
image of acceptable quality.
To check the opening;
a) remove one ocular and look down the tube,
b) adjust the condenser iris so that 80% of the field is light,
c) replace the ocular,
or
a) set condenser aperture iris scale to about 80% of the N.A. value of objective,
b) example: N.A. of objective is 0.75, set the scale to 0.75 x 0.8 = 0.6.
This Manual: http://www.manuallib.com/file/2605418