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INSTRUMENT DESCRIPTION
Objectives
Axioskop 2 / Axioskop 2
MOT
1-12
B 40-075 e 06/98
1.5 Objectives
The objectives are the optical centerpiece of the
microscope. The following is an example of how
objectives can be labelled:
Achroplan 10x/0.25
∞
0.17
where
10x
: objective magnification, with a defined
color ring on the objective being allocated
to each magnification step (Zeiss color
code)
0.25 : numerical aperture
∞
: infinite
image
distance;
these
objectives
can only be used with ICS microscopes
from Carl Zeiss
0.17 : can only be used with cover slip thickness
D = 0.17 mm
or
−
: can be used with cover slip thickness D = 0
or 0.17 mm
Other labels:
Oil
: oil immersion objective
Ph 2 : phase contrast objective with a green color ring and phase stop Ph 2
Color ring code for objective magnification
Color ring on
objective
black
brown
red
orange
yellow
green
light
blue
dark
blue
white
Magnification
factor
1.25x
2.5x
4x; 5x
6.3x
10x
16x;20x;
25x;32x
40x; 50x 63x
100x;
150x
The objective magnification multiplied with the eyepiece magnification (minimum 10x) results in the
visual overall magnification: e.g. 10 x 10 = 100x.
The numerical aperture x 1000, e.g. 0.25x1000 = 250x, is the highest useful magnification, i.e. no
further details are resolved above that limit.
The exact observance of the cover slip thickness of 0.17 mm is all the more necessary the higher the
numeric aperture of the objective. Therefore, certain objectives can be set for different cover slip
thickness (due to correction mount). For this, a specimen area is searched, and the position of the
correction ring where optimum focus and image contrast are obtained is determined (refocusing is
always required).
Immersion objectives are always insensitive to differences in cover slip thickness. When immersion
objectives are used, the air between the cover slip and the objective is replaced with a liquid, which is
Fig. 1-3
Objective
Summary of Contents for Axioskop 2
Page 1: ...Axioskop 2 Routine Microscope Operating Manual...
Page 14: ...INTRODUCTION Axioskop 2 Axioskop 2 MOT 0 14 B 40 075 e 06 98...
Page 120: ...OPERATION Axioskop 2 Axioskop 2 MOT 3 54 B 40 075 e 06 98...
Page 122: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 2 Axioskop 2 MOT 4 2 B 40 075 e 06 98...
Page 132: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 2 Axioskop 2 MOT 4 12 B 40 075 e 06 98...
Page 134: ...ANNEX Axioskop 2 Axioskop 2 MOT A 2 B 40 075 e 06 98...