Zeiss Axioskop 2, Manual, Page: 119 / 140

OPERATION
Axioskop 2 / Axioskop 2
MOT
Quantitative microscopy
B 40-075 e 06/98 3-53
Example:
Upper (3-34/ 1 ) and lower side (3-34/ 2 ) of a thin
section were focused using a dry objective
(n
M
= 1.0).
The indicated intervals of the mechanical fine drive
are 15.0 and 24.5, which means that d’ = 9.5 µm
with an interval of 1 µm.
The refractive index of the specimen area n
P
has
been assumed as n
P
= 1.5. Therefore, the thin
section height is:
d = d’ x = 9.5 µm x = 14.25 µm
10
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40
50
60
70
80
90
100
10
20
30
40
50
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Fig. 3-34 Height measurement
n
P
n
M
1.5
1