OPERATION
Axioskop 2 / Axioskop 2
MOT
Illumination and contrasting techniques
B 40-075 e 06/98
3-37
•
Bring SENARMONT polarizer (3-25/
7
), consisting of carrier with
λ
/4 plate (above) and rotary polarizer
(below), in click-stop position below the condenser, unless polarization filters are integrated.
•
Set luminous-field diaphragm and aperture diaphragm (3-25/
6
) in accordance with the KÖHLER rules.
•
Use knurled screw (3-25/
4
) on the DIC slider to set optimum contrast. When the DIC slider is moved
symmetrically around its center position, specimen details can be imaged in such a way that they
appear in three dimensions.
•
In SENARMONT DIC, the optimum contrast is set by turning the polarizer via the lever in the lower
part of the SENARMONT polarizer (3-25/
7
). The
λ
/4-plate above it must always be switched on for
SENARMONT contrasting to function. If the DIC slider was moved to the center position before, there
will be darkness in the stop position of the SENARMONT-polarizer, i.e. the path difference amounts to
0 nm in this position.
•
If required, the
λ
-compensator (3-25/
5
) can be inserted in the compartment for compensators above
the nosepiece to produce colored DIC contrast.
☞
Because of its use of polarized light, the DIC-technique is impaired if birefringent components,
e.g. foils sometimes used with histological sections, are positioned between polarizer and
analyzer. The same applies to Plexiglas culture chambers if the chamber is made of plastic. In
such cases, it is recommended to use chambers with glass bottoms to avoid the loss of optical
performance.
Summary of Contents for Axioskop 2
Page 1: ...Axioskop 2 Routine Microscope Operating Manual...
Page 14: ...INTRODUCTION Axioskop 2 Axioskop 2 MOT 0 14 B 40 075 e 06 98...
Page 120: ...OPERATION Axioskop 2 Axioskop 2 MOT 3 54 B 40 075 e 06 98...
Page 122: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 2 Axioskop 2 MOT 4 2 B 40 075 e 06 98...
Page 132: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 2 Axioskop 2 MOT 4 12 B 40 075 e 06 98...
Page 134: ...ANNEX Axioskop 2 Axioskop 2 MOT A 2 B 40 075 e 06 98...