Zeiss Axioskop 2, Manual

Page: 106 / 140

OPERATION
Illumination and contrasting techniques Axioskop 2 / Axioskop 2
MOT
3-40 B 40-075 e 06/98
•
Switch on the HBO 103 or HBO 50 mercury
vapor short-arc lamp (3-26/
2 ) and allow it to
heat up to its operation temperature for
approx. 15 mins.
•
Select the FL reflector module in reflector turret
(3-26/
1 ) containing the required fluorescence
filter combination (depending on the excitation)
and switch it on.
•
When using the incident-light filter slider
(3-26/
5 ), unblock the light path in the incident-
light illuminator.
•
Remove one eyepiece from the tube and set
the aperture diaphragm (3-26/
3 ) by opening it
until the entire objective exit pupil is unblocked.
•
Insert eyepiece in tube again and close
luminous-field diaphragm (3-26/
4 ) until it is
visible in the field of view.
•
Use the two centering screws (3-26/ 6 ) to center
the luminous-field diaphragm in relation to the
edge of the field of view.
•
Either open the luminous-field diaphragm until it just disappears behind the edge of the field of view
or, if there is a risk of specimen bleaching, reduce it until it is visible in the field of view.
•
Finally, refocus on the specimen and optimize the HBO 103 collector position as described in section
2.1.11. Set the collector in such a way that homogeneous illumination is obtained with the short-
wave excitation reflector module. When modules with long-wave excitation are used, correction of
the collector position is not required.
4
5
Fig. 3-26 Components for Epi-fluorescence
on the Axioskop 2