Zeiss Axioskop 2, Manual

Page: 94 / 140

OPERATION
Illumination and contrasting techniques Axioskop 2 / Axioskop 2
MOT
3-28 B 40-075 e 06/98
•
Insert eyepiece in the tube again.
•
If the height of the dark field condenser has
been set correctly and sensibly enough, any
visible brightening in the field of view can be
reduced, and the luminous-field diaphragm
image will be almost perfectly in focus.
•
Finally, match the diameter of the luminous-
field diaphragm to the size of the field of view.
☞
Darkfield microscopy requires specimens to be considerably cleaner than in other techniques.
Finger prints, dirt or dust particles in particular have negative effects, since they brighten the
background of the field of view and decrease the contrast of the object image.
Fig. 3-18 Centering of darkfield stop on the
universal condenser