Varian 450-GC, User Manual

Page: 80 / 205

AutoSamplers
Page: 124 User Manual 450-GC Varian, Inc.
Standard On-Column and Standard Split/Splitless Sampling Modes
Internal Standard i s no t permitt ed with Standard Sampling modes.
Standard mode parameters are set for samples dissolved in common solvents
such as iso-octane.
The Standard sampling mode for
On-Column
performs the following
sequence of actions:
Sampler
sequence
Standard On-column - mode
SyringeSize
(µL)
1 2 5 10 50 100 250
1
If used: Pre-Injection Solvent Clean Flushes
(solvent
→
waste)
- Volume (µL) 0.75 1.5 3.75 7.5 37.5 75 187.5
- Plunger Speed Up (µL/s) 0.5 1 2.5 5 10 20 50
- Plunger Speed Down (µL/s) 5 10 25 50 25 50 62.5
2
If used: Any Pre-Injection Sample Clean
Flushes
(sample
→
waste)
- Volume (µL) 0.75 1.5 3.75 7.5 37.5 75 187.5
- Plunger Speed Up (µL/s) 0.5 1 2.5 5 10 20 50
- Plunger Speed Down (µL/s) 5 10 25 50 25 50 62.5
3
Clean Strokes
(sample
→
sample)
- Plunger Speed Up (µL/s) 0.2 0.4 1 2 10 20 50
- Plunger Speed Down (µL/s) 5 10 25 50 25 50 62.5
- Volume For Fill Strokes (µL) 0.75 1.5 3.75 7.5 37.5 75 187.5
- Number Of Clean Strokes 5 5 5 5 5 5 5
- Viscosity Delay (s) 0.0 0.0 0.0 0.0 0.0 0.0 0.0
4
Draw Sample
- Plunger Speed During Fill (µL/s) 0.2 0.4 1 2 10 20 50
- Viscosity Delay (s) 0.0 0.0 0.0 0.0 0.0 0.0 0.0
Al l Cl eanStrokes - and Fl ush-Volumes are limit ed to 75% of the sy ringe
capacity.