AutoSamplers
Varian, Inc.
User Manual 450-GC
Page: 125
Sampler
sequence
Standard On-column - mode
SyringeSize
(µL)
1
2
5
10
50
100
250
5
Air Plug After sample
- Volume (µL)
0.1
0.2
0.5
0.5
0.5
2
4
6
Inject Sample
- Plunger Speed During Injection (µL/s)
0.5
1
2.5
5
5
10
12.5
- Pre Injection Delay (s)
0.0
0.0
0.0
0.0
0.0
0.0
0.0
- Post Injection Delay (s)
6.0
6.0
6.0
6.0
6.0
6.0
6.0
7
If used: Any Post Injection Solvent Clean
Flushes
(solvent
→
waste)
- Volume (µL)
0.75
1.5
3.75
7.5
37.5
75
187.5
- Plunger Speed Up (µL/s)
0.5
1
2.5
5
10
20
50
- Plunger Speed Down (µL/s)
5
10
25
50
25
50
62.5
Table 24: Injection mode parameters for On-Column
Internal Standard i s not permitted with Standard Sampling modes.
Standard mode parameters are set for samples dissolved in common solvents
such as iso-octane.
The Standard sampling mode for
Split/Splitless
performs the following
sequence of actions:
Sampler
sequence
Standard Split-/Splitless - mode
SyringeSize
(µL)
1
2
5
10
50
100
250
1
If used: Pre-Injection Solvent Clean Flushes
(solvent
→
waste)
- Volume (µL)
0.75
1.5
3.75
7.5
37.5
75
187.5
- Viscosity Delay (s)
9.9
9.9
9.9
9.9
9.9
9.9
9.9
- Plunger Speed Up (µL/s)
0.5
1
2.5
5
10
20
50
- Plunger Speed Down (µL/s)
5
10
25
50
25
50
62.5
2
If used: Any Pre-Injection Sample Clean
Flushes
(sample
→
waste)
- Volume (µL)
0.75
1.5
3.75
7.5
37.5
75
187.5
- Viscosity Delay (s)
9.9
9.9
9.9
9.9
9.9
9.9
9.9
- Plunger Speed Up (µL/s)
0.5
1
2.5
5
10
20
50
Al l Cl eanStr ok es- and Flush -Volumes are limi ted to 75% of t he syr inge
capacity.
Summary of Contents for 450-GC
Page 92: ...AutoSamplers Page 152 User Manual 450 GC Varian Inc...
Page 144: ...Detectors Flame Ionization Detector FID Page 294 User Manual 450 GC Varian Inc...
Page 177: ...Maintenance Page 438 User Manual 450 GC Varian Inc...
Page 179: ...Shipping Cleaning and Disposal Instructions Page 440 User Manual 450 GC Varian Inc...
Page 201: ......
