Varian 450-GC, User Manual

Page: 12 / 205

Table of Contents
Page: XII User Manual 450-GC Varian, Inc.
General laboratory precautions........................................................................................9
Operating precautions......................................................................................................9
Install Gas Regulators and Set Pressures..........................................................................10
Connect Gases...................................................................................................................10
Installing the CP-8400/8410 AutoSampler .........................................................................11
Connect GC to Power ....................................................................................................12
C
OLUMN
I
NSTALLATION AND
C
ONDITIONING
................................................................................13
Nuts and Ferrules for Capillary Columns............................................................................14
Connect Capillary Column to Injector .................................................................................15
Tips for Tightening Column Ferrules..............................................................................17
Nuts and Ferrules for Packed Columns..............................................................................18
Connect a Packed Column to the Injector..........................................................................18
Varian Test Column............................................................................................................19
Identify Carrier Gas Controls and Turn on Flow .................................................................19
Split Vent Flow and Septum Purge Flow............................................................................20
Septum purge calibration ...............................................................................................20
Set Carrier Gas Flow Rate to Condition Column................................................................22
Injector Conditioning...........................................................................................................22
Column Conditioning ..........................................................................................................23
New Columns .................................................................................................................23
Used Columns................................................................................................................23
Connect Capillary Column to Detector ...............................................................................24
Column Insertion Depths for Varian Detectors ...................................................................24
Connect Metal Packed Column to Detector .......................................................................25
Check for Leaks..................................................................................................................25
B
ASIC
O
PERATION
.....................................................................................................................26
Setting Carrier and Detector Gas Flow Rates ....................................................................26
Condition the GC System ...................................................................................................28
Build a Method to Analyze a Test Sample..........................................................................28
Method to Analyze a Varian Test Sample on a Varian Test Column .................................29
Prepare the Test Sample....................................................................................................30
Solvents for GC ..................................................................................................................31
Sample Volume and Injection Rate ....................................................................................32
Inject the Test Sample ........................................................................................................32
REFERENCE ..............................................................................................................................33
PERIPHERAL CONNECTORS...................................................................................................33
P
ERIPHERAL
C
ONNECTORS
........................................................................................................34
J504/J505 S.I.D. 1
AND
2.........................................................................................................35
J401 A
NALOG
OUT...................................................................................................................36
J302 T
EMPERATURE
P
ROBES
....................................................................................................37
J45/J46 A
UXILIARY
1
AND
2.......................................................................................................38
J44 C
RYO
V
ALVES
(3)...............................................................................................................39
J42/J43 E
XTERNAL
E
VENTS
......................................................................................................40
J41 H
EATER
C
ONNECTIONS
.......................................................................................................41
R
EAR
P
ANEL
............................................................................................................................42
O
PTION
B
OARD
.........................................................................................................................43
F
USES
C
OMMON
.......................................................................................................................44