Varian 450-GC, User Manual

Page: 35 / 205

Installation Installation
Page: Page: 16 16 User User Manual Manual 450-GC 450-GC Varian, Varian, Inc. Inc.
5. 5. Move the Move the nut and nut and ferrule to ferrule to within 3 within 3 cm of cm of column end. column end.
6. 6. Measure Measure the the mark mark point point (see (see Table 5 Table 5 ) from the end of the column and mark ) from the end of the column and mark
this distance with a felt this distance with a felt pen or typewriter fluid. The use pen or typewriter fluid. The use of Column Scale of Column Scale
tool (03-92575001, available in tool (03-92575001, available in Capillary Column Quick Connect Kit Capillary Column Quick Connect Kit on on
page 17 page 17 , 03-925751-90) or EZ-Grip , 03-925751-90) or EZ-Grip
tm tm
supplied with each standard GC supplied with each standard GC
capillary column is highly recommended. capillary column is highly recommended.
DO NOT allow the column nut and ferrule to fall past the mark DO NOT allow the column nut and ferrule to fall past the mark
becau
because the ink or se the ink or correction f correction f luid c luid c an contaminate the fe an contaminate the ferrule. rrule.
1041 1041 1061 1061 1177 1177 1079 1079 PTV PTV
1093 1093
On-Column On-Column
Insert Insert depth depth Maximum* Maximum* Maximum* Maximum* 3.7 3.7 cm cm 7.5 7.5 cm cm 8.6 8.6 cm cm
Mark Mark at at None None None None 2.7 2.7 cm cm 5 5 cm cm 5 5 cm cm
Table 5: Maximum insert depth for Capillary Column in Injector Table 5: Maximum insert depth for Capillary Column in Injector
7. 7. * Only for * Only for 1041 an 1041 and 1061In d 1061Injectors, for the jectors, for the other Injecto other Injectors continue rs continue at 12. at 12.
8. 8. Gently push Gently push the colum the column fully u n fully up into the p into the Injector (9 cm Injector (9 cm) until the ) until the column column
comes to a firm comes to a firm stop. This correctly positions the column against the upper stop. This correctly positions the column against the upper
end of the Injector insert. The column must go in the full distance. If it goes end of the Injector insert. The column must go in the full distance. If it goes
not, the Injector will
not, the Injector will not function properly. Tighten the column nut just not function properly. Tighten the column nut just
enough to prevent column movement. enough to prevent column movement.
9. 9. Manually in Manually insert the syrin sert the syringe that will ge that will be used be used with the co with the column into lumn into the 1041 the 1041
Injector, checking that the syringe goes into the Injector Injector, checking that the syringe goes into the Injector completely with no completely with no
binding or interference. If binding or interference. If interference occurs, repeat the above column interference occurs, repeat the above column
insertion procedure until the syringe moves smoothly into the Injector. insertion procedure until the syringe moves smoothly into the Injector.
10. 10. Continue to hold the column firmly in place as you tighten the column nut Continue to hold the column firmly in place as you tighten the column nut
carefully and only enough to hold the column firmly in place and obtain a
carefully and only enough to hold the column firmly in place and obtain a
good seal. Remove the syringe from the Injector.
good seal. Remove the syringe from the Injector.
11. 11. After the column is installed at the Injector end, make several dry injections After the column is installed at the Injector end, make several dry injections
to ensure that the needle enters the column easily.
to ensure that the needle enters the column easily.
12. 12. Partially insert the column into the lower end of the Injector. Thread up the Partially insert the column into the lower end of the Injector. Thread up the
capillary column nut and ferrule
capillary column nut and ferrule finger-tight. finger-tight.