Varian 450-GC, User Manual

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Table Table of of Contents Contents
Varian, Varian, Inc. Inc. User User Manual Manual 450-GC 450-GC Page: Page: XIX XIX
PULSED DISCHARGE HELIUM IONIZATION DETECTOR (PDHID).... PULSED DISCHARGE HELIUM IONIZATION DETECTOR (PDHID)..................................... .................................295 295
H H
ELIUM ELIUM
II
ONIZATION ONIZATION
(PDHID).... (PDHID)................................................................................................... ...............................................................................................296 296
Cable Cable overview overview .... ................................................................................................................. .............................................................................................................297 297
Exploded Exploded View View .... .................................................................................................................. ..............................................................................................................298 298
Gas Gas Connection Connections s .... .............................................................................................................. ..........................................................................................................299 299
Installation.... Installation......................................................................................................................... .....................................................................................................................299 299
General Precau General Precautions tions .... ......................................................................................................... .....................................................................................................300 300
Installing and Purging the Helium Purifier .... Installing and Purging the Helium Purifier .... .................................................................... ....................................................................300 300
Connecting the Discharg Connecting the Discharge Gas to e Gas to the Detector.... the Detector.... ............................................................ ............................................................301 301
Capillary Colum Capillary Column Connection n Connection .... ........................................................................................... .......................................................................................301 301
Packed Column Con Packed Column Connection nection .... ............................................................................................. .........................................................................................302 302
Testing Testing for L for Leaks eaks .... .............................................................................................................. ..........................................................................................................303 303
THERMIONIC S THERMIONIC SPECIFIC DETECTO PECIFIC DETECTOR (TSD) R (TSD) .... .......................................................................... ......................................................................305 305
Initi Initial Set-Up al Set-Up .... ..................................................................................................................... .................................................................................................................306 306
Operation and Operating Recommendation Operation and Operating Recommendations s.... ................................................................... ...............................................................307 307
Bead Condi Bead Conditioning tioning.... ........................................................................................................ ....................................................................................................307 307
Optimization of the TSD Using the Varian Test Samp Optimization of the TSD Using the Varian Test Sample le .... .............................................. ..........................................309 309
Determining Optimu Determining Optimum Hydrogen Flow m Hydrogen Flow .... ......................................................................... .....................................................................309 309
Maintainin Maintaining Optimum Cond g Optimum Conditions itions .... ................................................................................. .............................................................................310 310
Column Bleed.... Column Bleed............................................................................................................... ...........................................................................................................311 311
Solvent Solvent Consideratio Considerations ns .... ................................................................................................ ............................................................................................311 311
Chlorinated Chlorinated Solvents Solvents .... .................................................................................................... ................................................................................................311 311
Effect of Si Effect of Silylation Rea lylation Reagents gents .... ........................................................................................ ....................................................................................312 312
TSD TSD Assembly Assembly .... .................................................................................................................. ..............................................................................................................313 313
Expl Explode oded view d view .... .............................................................................................................. ..........................................................................................................313 313
TSD disa TSD disassem ssembly bly .... ......................................................................................................... .....................................................................................................314 314
TSD TSD Re-assembly Re-assembly .... ........................................................................................................ ....................................................................................................315 315
M M
AINTENANCE AINTENANCE
.... ......................................................................................................................... .....................................................................................................................317 317
Cleaning / Maintena Cleaning / Maintenance nce.... .................................................................................................... ................................................................................................317 317
Bead Bead Cleaning Cleaning .... .................................................................................................................. ..............................................................................................................317 317
Flame Tip and Internal Parts Clea Flame Tip and Internal Parts Cleaning ning.... ............................................................................. .........................................................................317 317
Replace the Flame-Tip Ferrule.... Replace the Flame-Tip Ferrule......................................................................................... .....................................................................................318 318
Test Chromatogram.... Test Chromatogram.......................................................................................................... ......................................................................................................318 318
THERMAL CONDUCTIVIT THERMAL CONDUCTIVITY DETECTOR (TCD) Y DETECTOR (TCD).... ..................................................................... .................................................................319 319
Before Oper Before Operating the TCD ating the TCD .... ................................................................................................ ............................................................................................320 320
Initi Initial Set-Up al Set-Up .... ..................................................................................................................... .................................................................................................................320 320
TCD Adjustm TCD Adjustments ents .... ......................................................................................................... .....................................................................................................322 322
Operatio Operation n .... .......................................................................................................................... ......................................................................................................................323 323
Range Setting.... Range Setting............................................................................................................... ...........................................................................................................325 325
Low Currents (100-150 mA).... Low Currents (100-150 mA)......................................................................................... .....................................................................................325 325
High High Currents (30 Currents (300 mA 0 mA)) .... ............................................................................................... ...........................................................................................326 326
Calculate Detector Sensitivity.... Calculate Detector Sensitivity....................................................................................... ...................................................................................327 327
Calculate De Calculate Detector Detectiv tector Detectivity ity .... ...................................................................................... ..................................................................................328 328
Adjust TCD Carr Adjust TCD Carrier Gas Flo ier Gas Flow Rates.... w Rates................................................................................. .............................................................................328 328
M M
AINTENANCE AINTENANCE
.... ......................................................................................................................... .....................................................................................................................328 328
TCD Test TCD Test Chromato Chromatogram gram .... ................................................................................................. .............................................................................................328 328