Teledyne CombiFlash EZ Prep, User Manual

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Section 5 Operation
5-5
5.1.3 Pre-loading on Column
You can load the reaction mixture or sample directly onto the
column, sometimes called “direct” or “dry” loading.
Note
If using this method, use care to ensure that a column equilibration
does not wash away the sample. Bypass the column equilibration
at run time by selecting “Preload on column” (or “No Equilibration”
for Rf 150 systems) as the sample Loading Type. Refer to Section
5.3 for more details.
5.2 Loading a Redi Sep Rf Column
To load a Redi Sep Rf column:
WARNING
The Combi Flash Rf has redundant safety devices to limit
pressure to less than 210 psi (1448 kPa). Redi Sep columns
smaller than 100 g are CE certified using standard IEC61010-1
for use on the Combi
Flash Rf. Redi Sep columns larger than
100 g meet Pressure Vessel Directive 97/23/EC. Teledyne Isco
strongly recommends against the use of columns rated less
than 210 psi (1448 kPa).
Note
For best results, always use Redi Sep Rf columns. The system will
not automatically detect other columns. The system also limits the
maximum operating pressure to 50 psi when the column is not
detected.
1. Select a Redi Sep Rf column. The on-line help includes a
column selection guide that can assist you in selecting a
stationary phase media and column size.
2. Raise the injection valve (column mount for Rf 150
systems) and insert the column into the top column mount.
Note that the column fittings are keyed to ensure the
correct flow direction.
3. Slowly lower the injection valve while aligning the bottom
column fitting. The spring-loaded injection valve will hold
the column in place.