Teledyne CombiFlash EZ Prep, User Manual

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CombiFlash® Rf
+
User Manual
6-58
possible that the detection absorbance trace alone would
indicate a single, valid chromatographic peak. In reality, it is a
combination of two peaks. By monitoring a second wavelength,
it may be possible to reveal the second compound. Because of
the slight shift in time and the different absorbance properties
of the two compounds, the changing ratio during the detected
peak would reveal the impurity. Therefore, one can therefore
assume that if the ratio is not constant for the entire duration
of the peak, the compound eluting may not be pure.
The SHOW SPECTRAL PURITY control measures purity by
using a comparison of UV-spectra measured at differing times.
The algorithm used is the "similarity index". The SPECTRAL
PURITY DETECTION button allows fractionation based on
spectral purity. The spectral purity algorithm doesn't will fail
on saturated peaks (flat on top due to detector saturation).
To display the ratio, open the M
ETHOD
E
DITOR [6.3.2]
window
and select the Show Ratio option.
6.4.10 Viewing runs
After completing a run, the PeakTrak Main window is used to
display all collected run data. You can also open previous runs
to view the chromatogram and the peak/tube locations. To
open a previous run:
1. From the main window, select File>Open, or click the Open
button. The F
ILES
[6.3.6]
window is displayed.
2.Choose a Run file and click the Open button.
3.PeakTrak displays the run in the M
AIN
W
INDOW [6.3.1]
.
The PeakTrak Main window will include:
• Rack and tube information — The left pane of the
window lists the current rack, a map for that rack, and a
table that lists the peaks and their corresponding tube
numbers. If the window is currently displaying the
collection parameters, click the DISPLAY RACK button
to view this information. On PurIon equipped systems,
the mass spectrum of the fraction contents can be
displayed by pushing the MS button and clicking on a
tube."