5-1
Combi
Flash
®
Rf
+
User Manual
Section 5 Operation
This section provides abbreviated operating instructions for
the Combi
Flash
Rf systems. For complete instructions, refer to
Help menu option from PeakTrak’s main menu.
5.1 Flash Sample Preparation
Before starting a run, consider how the sample will be
introduced to the column media. This section discusses three
methods: liquid injection, solid sample cartridges, and
pre-loading the sample on the column.
5.1.1 Liquid Sample Injection
If the sample is soluble in the starting mobile phase, it can be
prepared as a solution and injected onto the column when
prompted during the purification run.
5.1.2 Solid Samples
Some compounds are not soluble in solvents that are
compatible with the chromatography. In addition, the
compounds may have very limited solubility in any solvent,
resulting in sample volumes that are impractical for good
chromatography. The answer in this case is solid sample
introduction. Here the reaction mixture or dissolved sample is
mixed with the same media in the chromatography column.
Some samples of this type may be simply dissolved and placed
into a prefilled cartridge. You can then use the cartridge
immediately or dry it before placing it on the system.
Other samples may need more care. Typically you would
create a mixture of 20% sample load to media (w/w). Then dry
the mixture under conditions that will drive off the solvent
without affecting the compounds of interest. These compounds
remain bound to the media. Once dried, pour the
media/sample mixture into an empty cartridge.
Summary of Contents for CombiFlash EZ Prep
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