Teledyne CombiFlash EZ Prep, User Manual

Page: 153 / 245

CombiFlash® Rf
+
User Manual
6-32
particular compound by reducing fragmentation or adduct
formation.
• Mass Spectrum Graph - this displays the mass
spectrum. There are two mass spectra displayed. Blue
displays the maximum of the current injection. Red
displays the maximum of the previous injection for
comparison. When a new injection is performed, the blue
spectrum replaces the red spectrum and a new blue
spectrum is started. To inject a sample, move the PurIon
injection valve to the “Inject Sample” position.
Inject the sample (20 uL) and move the valve to the
“Scan Mass” position.
Note
Note:
Only use 22 gauge square tip needles (PN
29 ‐ 9001 ‐ 911) to avoid scratching the internal surfaces of
the injection valve. Filter the sample with a 0.45 µ filter to
avoid clogging the PurIon tubing and probe capillary.
Sample concentration should be less than 20 µg/mL.
• POLARITY - switches the mass spectrometer between
positive and negative polarity.
• FORMAT GRAPH - Set the displayed range of the
mass spectrum. The PurIon system still collects the
entire mass range even when the range is set within
than the minimum and maximum values allowed for the
spectrometer. Values can range from 50 to 1200 Da
(PURION and PURION S) or 2000 Da (PURION L).
Changing the mass range defined by these controls may
cause the Y-axis (ion count) scaling to change based on
the tallest peak within the range.
• Fluid Interface Pressure - The carrier solvent
pressure is displayed in a ribbon gauge. If the pressure
approaches the level of the red line, the system is
clogged and should be cleaned to allow proper operation.
• ION SETTINGS - Allows selection of different ion
settings to maximize the intensity of the desired
molecular ion. The factory selections include “Robust”
for compounds that do not easily ionize. “Typical” works
well for most compounds. The “Fragile” setting is used