Teledyne CombiFlash EZ Prep, User Manual

Page: 154 / 245

Section 6 User Help Reference
6-33
for those compounds that are delicate or easily fragment.
User-defined settings may also be loaded.
• MAKE CHANGES - Pressing this button allows a user
to change the settings to improve the intensity of an ion .
• SAVE- save changes to an existing ion settings file .
• SAVE AS…- save changes and create a new ion settings
file. This new file can be selected in ionization settings or
method development (see section 6.2.3.1). Additionally,
this file can also be selected when setting up a
separation run.
• FACTORY SETTINGS - restore the “typical”, “Robust”,
and Fragile” ion settings to the factory default values .
• PROBE - The probe nebulizes and ionizes the sample.
There is a choice of ESI (electrospray interface) or APCI
(atmospheric pressure chemical ionization) probes. The
software will change the labels on the control to reflect
the probe installed in the mass spectrometer.
• GAS TEMP -the temperature of the nebulization gas
for the probe. Lower temperatures are used for more
delicate, heat labile compounds. The temperature is
set to quickly evaporate the carrier solvent (note that
PURION S and PURION L systems will display a
single temperature for both positive and negative
ionization).
• VOLTAGE (CURRENT) - This displays a voltage
setting (ESI probes) or current value (APCI probes).
Lower values are used for more delicate compounds.
The capillary is heated to complete the evaporation of solvent.
It also carries a voltage; lower voltages are used for more
delicate compounds.
• Source Voltage - The source voltage settings have the
greatest effect on fragmentation. Higher values induce
more fragmentation but also reduce adduct formation.
• OFFSET - Offset is the voltage applied to all masses.
Large values tend to increase fragmentation but
reduce adduct formation.