Section 2 Preparation: Rf + , Rf + Lumen, Rf+ PurIon, and EZ Prep Systems
2-5
CAUTION
To prevent damage or premature wear to the pump and internal
valves, clean solvent should be used. The solvent should not
contain any dissolved solids.
CAUTION
If solvent containers are reused, particulate matter can
accumulate in the container. Containers should be thoroughly
cleaned before each refill. Failure to clean the containers may
result in damage to the solvent selection valve and internal
components.
The Combi
Flash
Rf
+
and Rf
+
Lumen systems have four
solvent inlet ports on the back panel, labeled 1-4.
The system requires at least one A and one B solvent to form a
binary gradient, and assumes that the B solvent is stronger
than A. The other inputs allow you to form alternative binary
gradient, or to change the B solvent mid-run.
The accessory package includes several pieces to complete the
solvent inlet connections. To connect the solvent lines:
1. Connect the solvent source to the system’s Solvent Inlet
ports. Figure 2-2 shows the recommended connections
when the user-supplied solvent container has a 38 or 45
mm opening (GL 38 or GL 45).
If the container does not have a GL38 or GL45 opening, do
not use the cap. Use an alternate method to cover the open-
ing, to prevent particulate matter from entering the con-
tainer. Ensure that the solvent and air tubing are secured
so that the weighted filter remains at the bottom of the sol-
vent source.
The Solvent Level Sensing air line connects to the back
panel with a compression fitting. This fitting must be air-
tight. To connect tubing using the compression fittings:
2. Slide the nut and ferrule onto the tubing as shown in
Figure 2-2.
3. Fully insert the tubing into the threaded bulkhead fitting
on the back panel of the system.
Summary of Contents for CombiFlash EZ Prep
Page 2: ...CombiFlash Rf User Manual ii ...
Page 8: ...CombiFlash Rf User Manual viii ...
Page 52: ...CombiFlash Rf User Manual 2 16 Figure 2 7 Loading test tubes ...
Page 85: ...CombiFlash Rf User Manual 3 20 ...
Page 103: ...CombiFlash Rf User Manual 4 18 ...
Page 185: ...CombiFlash Rf User Manual 6 64 ...
Page 199: ...CombiFlash Rf User Manual 7 14 ...
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