PerkinElmer aanalyst 200, User Manual

Page: 226 / 328

Analyzing Samples
8-16
Analyzing Samples Using Linear Calibration
The following example describes how to run samples using the linear calibration technique. It
should be noted that the same steps are carried out when using the nonlinear calibration
technique. The only difference is in setting up the calibration type. Instead of selecting nonlinear
for the calibration type, you select nonlinear.
EXAMPLE:
Determining copper using linear calibration
The procedures in this example assume that you have performed the set-up procedures in
Chapters 3, 4, 5, and 6, in which case the instrument will be on and the flame ignited.
This example runs a blank, 3 standards, and 3 test samples. We instructed the autosampler to
perform a reslope after the second test sample.
1. Carry out the following steps to duplicate our example:
2. Prepare solutions. Set up the autosampler
3. Load the autosampler
4. Enter parameters
5. Start the run
Detailed procedures follow.
Prepare solutions
In this example, we used a copper lamp and solutions of copper for our determinations. However,
you do not have to use copper. For your convenience in making substitutions we have included a
table of recommended concentrations for Ag, Mg, Mn, and Pb.
1. Prepare about 200-250 mL of 1% v/v HNO
3
to use as a blank solution.
2. Prepare about 200-250 mL each of three standards in 1% v/v HNO
3
of the element you want
to determine. Suggested standard concentrations for five elements are shown in the following
table: