110 | Chapter 3: Operation
Option
Description/Values range
Default
values
Positioning X- and Y- horizontal axes, Z- height (vertical) axis
can be adjusted to improve performance. See
Axis: Default Dispense and Aspirate Heights
Z = 336 for
96-well plate
X & Y = 0
Vacuum Delay
Suspends the vacuum pump until a certain volume is
dispensed. This feature is critical to cell wash
operations. It delays normal aspiration until the
specified volume has been dispensed to the wells.
The range is 0 to 30,000 µL/well.
0
More Options
Pre-dispense
Quick, small prime to condition the tips before
dispensing.
No
Bottom wash
Bottom washing adds an initial wash cycle to the
specified number of cycles. Fluid is simultaneously
dispensed and aspirated to create cleaning
turbulence (at the specified height). The manifold
descends to aspirate again and ends with a final
dispense to fill the wells.
No
Between cycles:
Shake
To mix the contents of the plate.
No
Duration From one second to one hour.
5 sec.
Intensity
Intensity
Variable
cycles through the other
levels of intensity
Slow
Medium
Fast
Medium
Soak
Delays wash for the duration to allow fluids in the
plate to steep or incubate.
No
Duration From one second to one hour.
30
Home carrier
To perform the shake or soak in the home position
or not. But, the plate carrier is moved home when
the total duration of the shake and/or soak exceeds
1 minute. The vacuum pump is turned off in this
scenario. Moving the plate home prevents
contaminating it with drops from the manifold.
No
Pre-dispense
between cycles
To wet or condition the manifold tubes between
cycles, which is only needed after a long soak.
No
MultiFlo™ FX Multi-Mode Dispenser