Perform the Peri-Pump Precision and Accuracy Tests | 225
Test
Volume
5 µL
5 µL
10 µL
10 µL
50 µL
50 µL
100 µL 100 µL
1536
6 µL
8-to-1
1120 µL
l
Set the
Flow Rate
to High for all protocols except the 1µL test; use the Medium
flow rate for this test.
l
Optionally, choose to
Require
the specific cassette type under test.
l
LHC/Touch screen users: select
Advanced options
: retain the default
Positioning
settings.
l
Define a
Pre-dispense
: set the volume to
10 µL
and the
Number of
Pre-dispenses
(cycles) to
2
.
l
RAD single-tube cassettes
: define the Well Map to dispense to one row only.
Then, perform the remaining steps in this procedure on that row only. Remember
to calculate the results based on 12 wells, rather than 96.
5. Place a clean/new microplate on the balance and tare the balance.
6. Put the Tube Organizer into the test fluid vessel and
Prime
the tubing until any
large air bubbles are removed.
7.
Run
the dispense protocol.
8. Place the plate on the balance and record the
Total Dispense Weight
in the
worksheet.
9. Using a calibrated hand pipette or the Peri-pump, add the specified amount of
deionized water to each well to raise the fluid level for optimal reading.
Test
Volume
1 µL
150 µL
5 µL
150 µL
10 µL
100 µL
50 µL
100 µL
100 µL 50 µL
1536
0 µL
8-to-1
0 µL
BioTek Instruments, Inc.
