184 | Chapter 4: Maintenance
Submerge and Shutdown
An overnight/multi-day maintenance option for soaking the tips and turning off
the instrument for overnight and weekend maintenance.
You can soak the MultiFlo FX dispense manifolds by filling the priming troughs
and turning off the instrument after the soak begins. The tubes will soak in the
priming troughs until the instrument is turned on again.
1. First, run
S-DAY_RINSE_A&B
but do not empty the priming trough inserts.
2. Turn off the instrument, and lower the manifolds into the priming trough.
3. Run
W-RINSE_AND_SOAK
.
4. When the wash manifold is submerged, turn off the instrument.
If the syringe dispenser manifolds do not lower themselves into their troughs,
gently push down on the dispense arm to submerge the manifold tips in the
priming fluid.
n
Remove either the cassette or the Peri-pump priming trough from the
instrument before performing this procedure.
5. Run a
to empty the washer's priming trough when
restarting the instrument.
n
Note: the washer aspirates fluid during the prime step, but it does not fully
evacuate the priming trough insert.
32-tube Syringe Dispenser
When using the 32-tube dispense manifold, especially the small bore (SB) model,
BioTek recommends using the following AutoPrime parameters to keep the
manifold clog-free:
Volume: 1000 µL
Flow rate: 3
Submerge duration: 3 hours (requires priming trough inserts)
Overnight/Multi-day practice: After modifying the AutoPrime parameters to
submerge the tubes for several hours:
1. Put the priming-trough-inserts into the priming trough.
2. Use
Quick Dispense
to fill both inserts (prime cups):
QUICK>PRIME
(for both
Syringe A and B). Run the
Prime
two or three times to fill the cups.
3. When the priming troughs are filled, press
Main Menu
.
MultiFlo™ FX Multi-Mode Dispenser
