Peri-pump Dispenser Maintenance | 193
The type of rinse fluid to use is determined by the type of fluid you are
dispensing. Some dispense fluids require the use of enzyme-active detergent,
buffered saline, ethanol or isopropyl alcohol, rather than deionized water alone.
Tools and Supplies
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Deionized or distilled water
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Buffered saline solution or enzyme-active detergent for protein or cell based
assays
At the start of the day:
Prime the tubing to prepare for a dispense run.
1. Reload the cassette and fill the supply vessel:
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When dispensing solutions not effected by water, simply prime with the
dispense fluid.
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When dispensing protein solutions, first prime the tubing with a buffered
saline solution to remove any traces of water in the tubing, then, prime with
the dispense fluid.
2.
Prime
the tubing until fluid flows into the priming trough and all visible air
bubbles have been removed.
At the end of the day:
Purge the tubing to reclaim the dispense fluid, then Prime the tubing to flush it
clean.
1.
Purge
the cassette until the tubing appears empty.
2. Replace the supply vessel with the appropriate rinse fluid:
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When dispensing water soluble solutions use deionized or distilled water.
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When dispensing protein solutions, first prime the tubing with a buffered
saline solution to remove protein particles, then, prime with deionized or
distilled water.
3.
Prime
the tubing for the specified duration:
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1 µL cassette = 5 seconds
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5 µL cassette = 7 seconds
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10 µL cassette = 10 seconds.
BioTek Instruments, Inc.