
Wash Parameters Table | 119
When a 'timer activated' delay is defined, Delay start and end events are added to
the protocol. The start and end events create a step container, similar to a Loop or
BioStack run.
The example above shows how to manage the start time of two sequential
operations, or steps. In this example, the timer is started prior to the Peri-pump
dispense operation and ended prior to the Syringe pump dispense operation. The
specified 5-minute delay is much longer than the duration of the Peri-pump
dispense operation.
Nested Delays
The timer activated delay can be nested within another time controlled series of
protocol steps. This may be necessary to specify multiple processing start times for
a complex series of steps, including loops and BioStack steps.
This example uses a BioStack rack of plates. The process requires that each plate in
the rack be filled with 250 µL from the Peri-pump and then, after 1 hour, the plate
is washed. The protocol will fill each plate in the rack, restack the plates, and then
wash each plate 1 hour after it was filled. The dispense and wash activities are each
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