Troubleshooting | 275
Problem
Possible Cause
What To Do
Volume too large for the
vessel.
Define a smaller volume.
Dispense rate too fast for
volume selected.
Define a slower dispense rate or
lower volume.
Uneven dispensing of
fluid;
wells not filled.
Clogged dispense tubes on
the dispenser manifold.
Clean the Syringe Dispenser
Manifold on page 201
Manifold or tubing not
adequately primed (air in
fluid lines).
Run a Prime using 20 mL. Follow
with a Dispense: 20
µ
L per well for
24 strips.
Dispense flow rate too
low.
Define a higher flow rate.
Setscrew not tightened on
the syringe pump piston.
Reinstall the syringe head as
described in
32-tube dispense
manifolds are not
dispensing accurately.
Calibrate the Backlash for Syringe
Dispenser on page 212
Highly viscous fluid.
Apply a
Pump Delay
to improve
performance when dispensing highly
viscous fluid, i.e. edit the dispense
or prime step to set a 500 msec.
delay.
Dripping dispense
tubes.
Dispense tubing routed
incorrectly.
The supply bottle tube must connect
to the Syringe’s bottom port.
Fluid jet is off-center
or skewed from 32-
tube SB manifold.
Minute particles of debris
on the end of the tubes.
Brush away any particles from the
end of the tube using a piece of
silicon tubing. Silicon will not flake
off and leave particles behind like
other materials.
BioTek Instruments, Inc.
