Biotek MultiFlo FX, Operator'S Manual

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130 | Chapter 3: Operation
plates to stagger the dilutions, as shown in the table below. Similarly, three
BioStack step blocks can result in a stack of plates sorted into dilution groups.
Using a series of Nth Plate or BioStack step blocks we can dispense 100 µL to
plates in the first pass, 200 µL to plates in the second pass, 300 µL in the third pass.
Choose the method that matches your preference for sorting the plates in the stack:
Group Plate
Nth
Plate Use
BioStack
A 1 100 µL 100 µL
2 200 µL 100 µL
3 300 µL 100 µL
B 4 100 µL 200 µL
5 200 µL 200 µL
6 300 µL 200 µL
C 7 100 µL 300 µL
8 200 µL 300 µL
9 300 µL 300 µL
Use an Nth Plate step to perform maintenance
Certain fluids, like BSA, can clog the liquid handler's dispense tubes, so flushing
the tubes after dispensing to a certain number of plates is recommended for
optimal performance. When using the BioStack you can use the Nth Plate step to
add a maintenance routine to the protocol.
Here's an example of using the Nth
Plate step to perform an AutoClean
loop after every 15 plates have been
washed. The Nth step is defined this
way:
Run only Nth Plate steps is
checked.
Remove plate before running steps is checked.
MultiFlo™ FX Multi-Mode Dispenser