182 | Chapter 4: Maintenance
Cassette Type Purge Volume Prime Volume
10 µL
250
500
5 µL
150
300
1 µL
50
100
Note: these volumes are per tube, except for the RAD cassettes, multiply times 8
for total volume. You may want to disable AutoPrime when trying to preserve
expensive reagents.
Turn on AutoPrime for the Syringe Dispenser
LHC
Touch screen
1. Select
Tools>Instrument
Utilities>AutoPrime
;
2. Set the
AutoPrime Interval
: enter the
downtime interval that will trigger an
AutoPrime.
3.
Fill the
Enabled
checkbox for the
dispenser.
4. Set the
Volume
and
Rate
. The default
settings are 400 µL at rate 3.
5. Click
Send
. Wait for a confirmation
message.
1. Select
Instrument
>AutoPrime
;
2. Set the
AutoPrime Interval
:
enter the downtime interval
that will trigger an
AutoPrime (for all devices).
3.
Fill the checkbox for the
dispenser and, then, press its
link.
4. Set the
Volume
and
Rate
.
The default settings are 400
µL at rate 3.
Note:
The
Volume
dispensed is actually double the input value. The Syringe dispenser
always performs two prime cycles during AutoPrime, doubling the volume. For example,
when 400 µL is entered, the dispenser actually primes the tubing with 800 µL.
You can
Submerge
the dispense tubes in the fluid
after
the
prime
, if desired. This
requires filling the
priming trough inserts
, one for each syringe. Each reservoir
holds approximately 6.5 mL.
After the dispense tubes have been primed, the manifold moves down into the
priming trough inserts. When both Syringes are enabled, the soak duration
begins after both dispensers are primed.
MultiFlo™ FX Multi-Mode Dispenser
