104 | Chapter 3: Operation
Step
Option
Description/Values range
Default
values
Prime
To remove air from the tubing.
Volume:
1-3000 µL
300
Duration:
1-300 seconds
3
Dispense
Volume
The per-well volume to dispense:
10
Up to
30,000 µL
per well is
supported.
1 µL cassette = 1 - 50 µL and 0.5 µL (HLF-D) for some
models*
5 µL cassette = 5 - 2500 µL
10 µL cassette = 10 - 3000 µL
Flow rate:
The rate, μL/second/tube, that fluid is dispensed for
each cassette type:
Cassette Type
1 μL
5 μL
10 μL
(μL/sec/tube)
Low
50
120
140
Medium
60
140
160
High
64
160
180
RAD
technology: Single-channel cassette flow rates
match the standard cassettes and the 8-to-1 cassette
matches the 10 µL flow rates.
When dispensing 0.5 µL with the 1 µL cassette the
average flow rate values are:
1 μL Cassette
0.5 μL Dispense
(μL/sec/tube)
Low
52
Medium
54
High
56
High
Cassette
type
require
To require a specific cassette type for this protocol. If
yes, select the type, if no, select ANY.
ANY
MultiFlo™ FX Multi-Mode Dispenser