Perform the Syringe Dispense Precision & Accuracy Test | 236
Syringe Dispenser Test Materials
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Microplates: 384-well plates for testing the 16-tube dispensers (which can be replaced
with 96-well plates if more applicable for your lab); 1536-well plates for testing the 32-
tube dispensers; and 96-well plates for testing the 8-tube dispensers. For the low-
density-plate-type specific manifolds, the applicable plate type is required.
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Precision balance with readability of 0.0001 g resolution is preferable, 0.001 g resolution
is acceptable, and capacity of 100 g minimum
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Pipettes and graduated beakers
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Microplate absorbance reader capable of dual wavelength reading at 630 and 405 (or
450) nm
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The test solutions:
Syringe Dispenser Test Solutions below
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Important Recommendations for All Liquid Tests on page
Syringe Dispenser Test Solutions
20 µL Solution
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Mix 10 mL of BioTek’s blue dye solution with 100 mL of deionized or distilled water
to create a dilution of the concentrate. Mix 160 mL of deionized or distilled water
with 20 mL of the diluted concentrate.
80 µL Solution
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Mix 120 mL of deionized or distilled water with 40 mL of the
20 µL Solution
.
1536 Solution
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Mix 21 mL of 70% Isopropyl Alcohol with 13 mL of the
20 µL Solution
and 150 mL
of DI H2O.
Perform the Syringe Dispense Precision & Accuracy Test
Prerequisites:
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Gather the required materials:
Syringe Dispenser Materials above
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Prepare the test solutions:
Syringe Dispenser Test Solutions above
.
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Make a copy of the applicable worksheets.
BioTek Instruments, Inc.
