254 | Chapter 5: Qualification
6. When the protocol is completed, carefully remove the plate. Place the plate on
the balance and record the
Total Dispense Weight
.
7. Read the plate in an optical reader (blank on air), using the dual-wavelength
method (630 nm - 450 nm), then print or export the results.
8. Use the
Dispense Precision Test Worksheet
to perform data reduction:
Tip:
If you have a spreadsheet software program, enter/export all 96 values into a
spreadsheet and apply your program’s Standard Deviation function (e.g., Microsoft
®
Excel’s STDEV).
a. Calculate the
Standard Deviation
.
b. Calculate the sum of the OD values for all wells, then divide by
number of
wells
, e.g.
96
, to determine the
Mean OD
for the plate.
c. Calculate the
%CV
: (Standard Deviation / Mean OD) * 100.
The %CV should be
<= 4.0
,
except for 6-well plates the %CV should be
<= 6.0
.
If the %CV is
greater than
3.0, one or more dispense tubes may need to be cleaned.
Remove the manifold and use the stylus to clean the dispense tube(s) giving lower-
than-average absorbance readings. When finished, re-prime the washer and retry
the test.
9. When finished, prime with deionized water to flush out the dye solution.
Evacuation Efficiency Test
n
Save the plate!
When this test is complete, use the plate to perform the
Shake Test for the OQ, if applicable.
n
Prerequisite for plate types other than 96-Well
: Create the protocols
See Create Washer Qualification Protocols
. One time only! (Predefined protocols are provided with the
instrument qualification package (PN 1260521).
n
If you tared the balance at the start of the
Dispense Precision and
Accuracy Test
, use the plate from that test here; skip steps 1-3.
1. Fill a supply bottle with 2 liters of deionized or distilled water. Run the
Maintenance program
W-DAY_RINSE
two or three times to prime the tubing
and manifold.
MultiFlo™ FX Multi-Mode Dispenser