252 | Chapter 5: Qualification
* BioTek Solution #1 100X Concentrate Wetting Agent 125 mL (PN 7773002)
contains 10% Tween 20 in deionized water and 0.01% Sodium Azide as a
preservative.
SOLUTION #2: Residual Test Solution
Mix 100 mL of
Solution #1
with 0.050
grams of FD&C #1 blue dye.
or
Mix 90 mL of
Solution #1
with 10 mL
of BioTek Blue Test Dye*.
* BioTek Solution #2 10X Concentrate Blue Test Dye 125 mL (PN 7773001)
contains 5 grams per liter FD&C Blue #1, 0.1% Tween 20 in deionized water and
0.01% Sodium Azide as a preservative.
SOLUTION #3: Dispense Precision and Accuracy Solution
Mix 1180 mL of deionized or distilled water with 20 mL of
Solution #2
.
Create Washer Qualification Protocols
1. Select
Maintenance
at the Home screen, and then
Define
.
2. Press
Create
.
3. Press the
Name
field and assign a unique name, e.g. 6-Well
Dispense Test.
4.
Plate Type
: select the appropriate plate type for the test and
press
OK
.
5.
Add
steps with the recommended parameters (shown below):
l
Add a
W-Dispense
step for the Dispense Precision and
Accuracy test and
W-Aspirate
step for the Evacuation
Efficiency test.
l
Press
Advanced Options
to define the Z-axis, Pre-Dispense,
and other parameters.
6. Press
Save
to save the protocol for future use.
Dispense Precision and Accuracy Protocols
Parameters
6-Well
12-Well
24-Well
48-Well
384-well
Volume (µL/well)
5560
2240
1120
560
80
MultiFlo™ FX Multi-Mode Dispenser
