Performance Specifications | 23
Low-Density-Plate-Specific Manifolds
Dispense Precision
Refer to the values below for dispense precision when measured in full plates with the
specified volume of deionized water with 0.1% Tween 20 and FD&C#1 blue dye solution
and read at 630 nm/405 nm.
Plate Type
Performance
Volume (µL/well)
6-Well
≤5.0% CV
5560
12-Well
≤3.0% CV
2240
24-Well
≤3.0% CV
1120
48-Well
≤3.0% CV
560
Dispense Accuracy
is measured gravimetrically when dispensing any volume of room-
temperature deionized water using
Flow Rate
2. For all plate-specific manifolds, dispense
accuracy shall be 2 µL or 1%, whichever is greater.
Strip Washer
Residual Volume (Evacuation Efficiency)
96-
Well
Average residual of ≤2.0 µL per well after a 3-cycle wash when dispensing 300
µL per well using a bottom-touching well aspiration and a solution of deionized
water with 0.1% Tween® 20 or equivalent buffer solution. (The aspirate height
adjustment should be optimized for the plate prior to testing.)
6-, 12-,
24-,
48-
Well
Refer to the table below to determine the average residual volume per well after
dispensing the specified µL-per-well using a bottom-touching well aspiration and
a solution of deionized water without Tween in flat-bottomed plates. (The
aspirate height adjustment should be optimized for the plate prior to testing.)
Washer Manifold
Volume (µL/well)
Travel Rate
Residual Volume (µL/well)
6-well
5560
0 CW
600
12-well
2240
0 CW
150
24-well
1120
0 CW
50
48-well
560
0 CW
25
BioTek Instruments, Inc.